虫生真菌中对松墨天牛高毒性蛋白的筛选及性质测定  

Screening and characterization of proteins from entomogenous fungi toxic to Monochamus alternatus (Coleoptera: Cerambycidae)

作　　者：李建庆[1] 张永安[1] 张星耀[1] 袁锋[2] 王玉珠[1] 

机构地区：[1]中国林业科学研究院森林生态环境与保护研究所 [2]西北农林科技大学植物保护学院,陕西杨凌712100

出　　处：《昆虫学报》2005年第1期1-6,共6页Acta Entomologica Sinica

基　　金：国家重点基础研究发展规划"973"项目"林木育种的分子基础"(G19990 160 )

摘　　要：从 6株虫生真菌中分离纯化了 4 8种蛋白。以松墨天牛Monochamusalternatus为试虫 ,通过生测筛选出 4种高毒性蛋白 ,并从 4种高毒性蛋白中筛选出毒力最强的蛋白Bb36W D。蛋白Bb36W D含 2个亚基 ,大亚基和小亚基的分子量分别约为 2 4kD和 17kD ;等电点分别为 9 4 7和 9 32。该蛋白含 16种氨基酸 ,其中精氨酸、组氨酸及丙氨酸含量较高。Forty eight proteins were purified from six strains of entomogenous fungi. Four high toxic proteins were screened by bioassay, with Monochamus alternatus larvae as the targets. The further screening of 4 high toxic proteins showed that the toxicity of protein Bb36W-D was the highest in all the proteins. Protein Bb36W-D is composed of two subunits of different sizes. Molecular weight is calculated to be 24 kD for the big subunit and 17 kD for the small subunit. Isoelectric point is 9.47 for the big subunit and 9.32 for the small subunit, respectively. Protein Bb36W-D is composed of 16 ammonia acids. Concentrations of arginine, histidine and alanine are higher than others.

关 键 词：虫生真菌 松墨天牛 毒蛋白 筛选 分子量 等电点 

分 类 号：Q962[生物学—昆虫学]