新生鼠海马缺氧缺血再灌注后p-CREB、c-Jun与神经保护  

Relationship of Neural Protection and Expression of P-CREB and C-Jun in Hippocam pal of Neonatal Rats after Hypoxia-Ischemia Brain Damage and Reperfusion

作　　者：任广立[1] 任瑞霞[2] 白雪帆[1] 王玲[3] 王茂贵[3] 

机构地区：[1]第四军医大学唐都医院感染科,西安710038 [2]济宁第一人民医院检验科,济宁272111 [3]第四军医大学唐都医院儿科,西安710038

出　　处：《实用儿科临床杂志》2005年第2期133-135,i001-i002,共5页Journal of Applied Clinical Pediatrics

摘　　要：目的　探讨缺氧缺血再灌注后海马神经元的存活机制。方法　 5 6只SD 7日龄鼠随机分入假术对照组、缺氧缺血脑损伤 (HIBD)组。制备HIBD模型。多普勒监测动脉血流。免疫组织化学测磷酸化的CREB、c Jun在海马区的表达。Thionin染色观察神经元凋亡。结果　HIBD再灌注 3、2 4h仔鼠右侧海马各区p CREB达高峰 ,7d后降至对照组水平 ;对照组两侧p CREB有基础表达 ,但无差异。c Jun 6h达高峰 ,2 4h稍降 ,48h又升高 ,7d后显著降低 ,但仍显著高于对照组 (P <0 .0 1) ;对照组两侧低表达。再灌注 2 4h右侧海马CA1区神经元已有明显凋亡 ,但 7d后神经元无明显丢失。假手术组海马极少数神经元凋亡。结论　CREB磷酸化可能经信号转导调节c Jun的表达促进神经元损伤后修复。这对保护CA1区锥体神经元是非常重要的。Objective To explore the survival mechanism of hippocampal ne urons after damage of hypoxia-ischemia and reperfusion of brain.Methods Seven days old SD rats(n=56) were randomly divided into hypoxia-ischemia br a in iniury(HIBD) group and sham group.The HIBD and reperfusion model was establis hed.The flowing of blood was de tected by multicolor Doppler.The p-CREB(phosphorylated c-AMP response element bi nding protein)and c-Jun were immunohistochemically evaluated in hippocampus.Thi onin staining was used to observe the apoptosis.Results The expression of p-CREB reache d the peak at 3,24 h postreperfusion in the right hippocampus of HIBD group,and then decreased to the normal level on the 7th day.In contral group the same reg ions showed basic immn-noreactivity.While c-Jun reached the peak at 6 h postreperfusion,then with a slightly decrease at 24 h;and at 48 h the other peak appeared,then with a gradual decline .On the 7th day the mumber of positive cells were still significanthy more than control group(P< 0.01).The control group showed low immunopositivity.The neurons of right hippocampus showed the character of apoptosis at 24 h postreper fusion.But on the 7 d of recirculation the loss of neurons had no obvioous disti nction (P>0.05).The sham animal showed very few apoptosis cells in the regio ns of hippocampus.Conclusions The persistent activation of CREB in the hippocampus regulates,the expression of c-Jun through the signal transductions and is involved in the course of neuron s′ survival and repair during the period of post hypoxia-ischemia reperfusion.I t is very important for the protection of the pyramidal hippocampal neurons on t he damaged side,especially for the sensitive region CA1. J Appl Clin Pediatr,2005,20(2):133-135

关 键 词：脑缺氧 脑缺血 再灌注 c-AMP反应元件结合蛋白 

分 类 号：R722.1[医药卫生—儿科]