HPLC-ELSD法测定银杏叶软胶囊中萜类内酯的含量  

Determination of terpenelactones in Yinxingye Soft Capsule by HPLC-ELSD

作　　者：张亚中[1] 周亚球[2] 王娟 陶建生[1] 

机构地区：[1]上海中医药大学,上海201203 [2]安徽中医学院,安徽合肥230038 [3]南京医药合肥天星有限公司,安徽合肥230001

出　　处：《中成药》2005年第1期22-24,共3页Chinese Traditional Patent Medicine

摘　　要：目的测定银杏叶软胶囊中萜类内酯的含量。方法采用高效液相色谱法蒸发光散射检测器(HPLCELSD)测定银杏叶软胶囊中萜类内酯的含量。色谱条件为汉邦LichrosperC18柱,4.6mm×150mm;正丙醇四氢呋喃水(1∶15∶84)为流动相;蒸发光散射检测器检测参数漂移管温度115℃;氮气流速3.5L·min-1。结果该方法简便易行,银杏叶软胶囊中基质对萜类内酯的测定无干扰,线形关系良好;萜类内酯的重现性试验RSD为1.04;平均加样回收率为97.1。结论本方法可作为银杏叶软胶囊中萜类内酯成分质量控制的一种有效方法。AIM: To determine the content of terpenelactones in Yinxingye Soft Capsule(Folium Ginkgo). METHODS: HPLC with Evaporative Light Scattering Detector (ELSD) was established to determine the content of terpenelactones in Yinxingye Soft Capsule. The analysis was carried out on C 18 column. The mobile phase was 1-propanol-THF-water(1∶15∶84). The operating conditions of the ELSD were a nebulizer nitrogen flow-rate of 3.5L·min -1 and an evaporator tube temperature of 115℃. RESULTS: No interference was found in the terpenelactones peak position. The method was simple, with a good linear relationship. The relative standard deviation of reproducibility was 1.04% and the average recovery was 97.1%. CONCLUSION: The method can be used for quality control.

关 键 词：银杏叶软胶囊 萜类内酯 高效液相色谱 蒸发光散射检测器 

分 类 号：R927.2[医药卫生—药学]