莪术油微球的含量测定  

Determination of Zedoary turmeric oil microsphere

作　　者：游剑[1] 于英伟[1] 李青坡[1] 崔福德[1] 

机构地区：[1]沈阳药科大学药剂教研室,辽宁沈阳110015

出　　处：《中成药》2005年第1期25-28,共4页Chinese Traditional Patent Medicine

摘　　要：目的研究建立莪术油固体化微球的含量测定方法。方法将莪术油固体化微球溶剂超声提取,用香草醛浓硫酸液显色后,采用可用可见分光光度法对制剂中莪术油进行含量测定;采用HPLC法运用二极管阵列检测器同时测定提取液莪术油中3种有效成分含量。结果运用可见分光光度法测定5批微球包油率均在60以上;运用HPLC法测定微球中含莪术二酮、莪术醇、吉玛酮平均分别为6.88、0.95、5.0。结论方法学考察可知两种方法准确、可靠;可见分光光度法简便,适用于微球中总油的含量测定,HPLC法则适用于本制剂化学成分的定性定量分析。AIM: To study a method for the determination of Zedoary turmeric oil microsphere (ZTO-MS). METHODS: ZTO-MS was extracted supersonically by solvent and then colored by sulfuric acid-vanillin reagent. The absorbance was measured by visible-spectrophotometry at 508nm. At the same time, ZTO-MS also could be determined by HPLC. RESULTS: The content of Zedoary turmeric oil was above 60% and the contents of Curdione、Curcuma、Germacrone were 6.88%,0.95%,5.0%, respectively, in ZTO-MS. CONCLUSION: Two methods for the determination of ZTO-MS are accurate, reliable; the method of visible-spectrophotometry is convenient and appropriate for determination for content of oil in ZTO-MS, while the method of HPLC gains the advantage for qualitative and quantitative analysis of components in ZTO-MS.

关 键 词：莪术油 微球 分光光度法 HPLC 

分 类 号：R927.2[医药卫生—药学]