HPLC同时测定复方石韦片和苦参药材中苦参碱和氧化苦参碱的含量  

Determination of oxymatrine and matrine in Compound Shiwei Tablets by HPLC

作　　者：江维克[1] 王丽 

机构地区：[1]贵阳中医学院药学系,贵州贵阳550002 [2]麦迪科医药科技开发研究所,贵州贵阳550002

出　　处：《中成药》2005年第1期45-48,共4页Chinese Traditional Patent Medicine

摘　　要：目的建立苦参药材及其制剂——复方石韦片(石韦、苦参、黄芪、篇蓄等)中氧化苦参碱和苦参碱的含量测定方法。方法采用高效液相色谱法,使用HypersilC18ODS2色谱柱,以甲醇0.2三乙胺溶液(45∶55)为流动相,流速为1.0mL·min-1,检测波长为220nm。结果氧化苦参碱在20.7～207.0μg范围内呈良好的线性关系(r=0.9998),在苦参药材及制剂中的平均回收率分别为101.0和100.3,RSD为1.5和0.67;苦参碱在20.7～207.2μg范围内呈良好的线性关系(r=0.9999),在药材及制剂中的平均回收率分别为101.2和100.6,RSD为1.2和0.74。结论本法灵敏、准确、专属性强,重现性好,可作为苦参药材及其制剂的质量控制方法。AIM:To establish an assay of oxymatrine and matrine in Compound Shiwei Tablets(Folium Pyrrosiae, Radix Sophorae Flavescentis, Radix Astragali, Herba Polygoni Avicularis, etc.) and Radix Sophorae Flavescentis by HPLC. METHODS: Diamonsil C 18 (10 μm,250 mm×4.6 mm) analytical column was used.The mobile phase consisted of methanol-0.2% phosphoric acid solution (45∶55). The detection wavelength was 220nm. RESULTS : The average recoveries of oxymatrine and matrine in Compound Shiwei Tablets were 100.3% and 100.6% , respectively and in Radix Sophorae Flavescentis were 101.0% and 101.2%, respectlively. CONCLUSION: This method could be used for the quality evaluation of Compound Shiwei Tablets and Radix Sophorae Flavescentis.

关 键 词：苦参 复方石韦片 氧化苦参碱 苦参碱 HPLC 

分 类 号：R927.2[医药卫生—药学]