大孔吸附树脂分离纯化四逆散活性部位的研究  

Separation and purification of active fraction with macroporous resigns

作　　者：李春成[1] 杨星昊[1] 崔敬浩[1] 王燕飞[1] 朱佳[1] 

机构地区：[1]南京师范大学新药研究中心,江苏南京210097

出　　处：《中成药》2005年第1期84-88,共5页Chinese Traditional Patent Medicine

基　　金：江苏省新药创制发展基金编号:021220

摘　　要：目的应用大孔吸附树脂纯化四逆散的水煎液,以期得到与活性部位成分组成相似的纯化产物。方法从主要成分含量、指纹图谱、总固形物得率、除杂试验等多角度不同层次比较分析不同纯化产物与活性部位成分群的相似度和纯化效果。结果HP20大孔吸附树脂的乙醇洗脱液具有与活性部位相似的成分组成。结论HP20大孔吸附树脂对该复方的活性成分分离效果好,吸附容量大,易洗脱。AIM: To purify the water-extract of Sinisa recipe with macoroporous resigns. METHODS: Screening the purification condition by means of the content of active principle, fingerprint chromatography, the yield of dry extracts,et al. of the purified product. RESULTS: The purified product by type HP20 macoroporous resign is in good agreement with the active fraction of Sinisa in the composition of specific principles. CONCLUSION: HP20 type macoroporos resign is considered as optimum for active fraction of Sinisa recipe in purifying efficience.

关 键 词：活性部位 分离纯化 大孔吸附树脂 

分 类 号：R284.2[医药卫生—中药学]