Sabin2型脊髓灰质炎病毒适应人胚肺二倍体细胞不同代次病毒滴度与5′端非编码区变异关系  

Mutations of 5′NCR and the Infectious Titers of Different Passages of Poliovirus Sabin 2 in Human Embryonic Lung Diploid Fibroblast (KMB17)

作　　者：胡凝珠[1] 瞿素[1] 刘国栋[1] 姬秋彦[1] 胡云章[1] 

机构地区：[1]中国医学科学院医学生物学研究所中国协和医科大学,昆明650118

出　　处：《病毒学报》2005年第1期65-68,共4页Chinese Journal of Virology

摘　　要：To investigate the adaptation of poliovirus Sabin 2 in human embryonic lung diploid fibroblast（KMB17） and its relation to nucleotide mutations of 5′ noncoding region（5′NCR）,the Sabin 2 vaccine virus was passaged serially in KMB17 for seventeen passages.The infectious titers of Sabin 2 virus increased along with the passages,the highest titer reached 8.0 LgCCID50/ml at passage 13（P13）.During passage,there were three nucleotide mutations appeared in 5′NCR,G changed to C at position 20,C to U at position 189 and G to A at position 481.The results showed that Sabin 2 poliovirus adapted well in KMB17 after 17 passages.The nucleotide mutation at position 481 that appeared from passage 3 on indicated the possibility of virulent reversion.To investigate the adaptation of poliovirus Sabin 2 in human embryonic lung diploid fibroblast(KMB17) and its relation to nucleotide mutations of 5′ noncoding region(5′NCR),the Sabin 2 vaccine virus was passaged serially in KMB17 for seventeen passages.The infectious titers of Sabin 2 virus increased along with the passages,the highest titer reached 8.0 LgCCID_(50)/ml at passage 13(P13).During passage,there were three nucleotide mutations appeared in 5′NCR,G changed to C at position 20,C to U at position 189 and G to A at position 481.The results showed that Sabin 2 poliovirus adapted well in KMB17 after 17 passages.The nucleotide mutation at position 481 that appeared from passage 3 on indicated the possibility of virulent reversion.

关 键 词：Sabin2型脊髓灰质炎病毒 感染性滴度 5′端非编码区 人胚肺二倍体细胞 适应性传代培养 变异 

分 类 号：R373.22[医药卫生—病原生物学]