妊娠高血压综合征及妊娠期糖尿病孕妇血小板功能状态的研究  

Study on the variation of platelet function in pregnancy induced hypertension and gestational diabetes mellitus

作　　者：尹松梅[1] 李益清[1] 谢双锋[1] 马丽萍[1] 吴裕丹[1] 聂大年[1] 冯坚红[1] 徐立卓[1] 

机构地区：[1]中山大学附属第二医院血液内科,广州510120

出　　处：《中华妇产科杂志》2005年第1期25-28,共4页Chinese Journal of Obstetrics and Gynecology

基　　金：广东省科技计划基金资助项目 (2 0 0 3C3 2 70 9)

摘　　要：目的　探讨妊娠高血压综合征 (妊高征 )及妊娠期糖尿病孕妇血小板功能状态和血小板活化情况。方法　选择妊娠晚期中度妊高征孕妇 2 1例 (妊高征组 ) ,妊娠期糖尿病孕妇 2 3例 (妊娠期糖尿病组 ) ,正常妊娠晚期妇女 2 0例 (对照组 )作为研究对象。采用血细胞分析仪和流式细胞术观察3组孕妇血小板计数、血小板平均容积和血小板α 颗粒膜蛋白 (CD6 2P)的表达。结果　 (1)妊高征组孕妇血小板计数为 (181± 5 6 )× 10 9/L ,妊娠期糖尿病组孕妇为 (2 0 6± 6 0 )× 10 9/L ,对照组孕妇为 (2 2 9± 5 6 )× 10 9/L。妊高征组孕妇血小板计数显著低于对照组 ,两组比较 ,差异有统计学意义 (P <0 0 1) ;妊娠期糖尿病组与对照组比较 ,差异无统计学意义 (P >0 0 5 )。 (2 )妊高征组孕妇的血小板平均容积为 (11 2± 2 0 )fl,妊娠期糖尿病组孕妇为 (9 5± 1 6 )fl,对照组孕妇为 (8 7± 1 6 )fl,妊高征组血小板平均容积高于对照组 ,两组比较 ,差异有统计学意义 (P <0 0 0 1)。而妊娠期糖尿病组血小板平均容积与对照组比较 ,差异无统计学意义 (P >0 0 5 )。 (3)妊高征组孕妇阳性血小板百分比CD6 2P(% )为 (42± 13) %、阳性血小板荧光强度 [CD6 2P(I) ]为 10 9± 39,妊娠期糖尿病组孕妇血小板CD6 2P为 (42± 14 ) %Objective To investigate the platelet activity and function in pregnancy induced hypertension (PIH) and gestational diabetes mellitus(GDM) Methods Twenty one patients with GDM and 23 patients with PIH in third trimester were included Twenty normal pregnant women in third trimester served as controls Platelet count (PC), mean platelet volume (MPV) were determined on Cell DYN 1600 and the expression of CD62P was analyzed on FACSC alibur Results (1) PC was (181±56)×10 9/L in PIH , (206±60)×10 9/L in GDM and (229±56)×10 9/L in controls, respectively PC in PIH was lower than that of controls ( P <0 01), but there was no significant difference between GDM and controls (2) MPV was (11 2±2 0) fl in PIH, significantly higher than that of controls (8 7±1 6) fl ( P <0 001) In GDM, MPV was (9 5±1 6) fl, without significant difference compared with that of controls (3) The expression of CD62P increased significantly in PIH compared with controls [CD62P:(42±13) % vs (26±7) %, P <0 001; CD62P(I):109±39 vs 75±13, P <0 01] In GDM, the expression of CD62P also increased significantly compared with the normal pregnancy[CD62P(%):(42±14)% vs (26±7), P <0 001; CD62P(I):100±42 vs 75±13, P <0 05] (4) All parameters had no significant difference between PIH and GDM Conclusion Platelet activity is enhanced in PIH and GDM It may play an important role in the pathogenesis and development of the two diseases

关 键 词：妊娠高血压综合征 妊娠期糖尿病 孕妇 血小板活化 血细胞分析仪 流式细胞术 

分 类 号：R714.25[医药卫生—妇产科学]