La(Ⅲ)-H_2O_2模拟酶体系催化PNPP水解的动力学研究  

Kinetic Investigation on the Hydrolysis of PNPP Catalyzed by La^(3+)-H_2O_2 Compound

作　　者：蒋桃艳[1] 叶小燕[1] 

机构地区：[1]乐山师范学院环境与生命科学系,四川乐山614004

出　　处：《乐山师范学院学报》2004年第12期57-59,共3页Journal of Leshan Normal University

基　　金：乐山师院学生科研资助课题

摘　　要：本文研究了在30±0.1℃,,La(Ⅲ)-H2O2体系的置留时间及配比,表面活性剂CTAB与Brij35,pH值对La(Ⅲ)-H2O2体系催化水解PNPP的影响。实验结果表明,置留时间为45分钟,[La(Ⅲ)]:[H2O2]=1:3,pH=7.00时,活性物种的催化活性最大,其水解速率常数Kobsd为3.85×10-3s-1,而加入表面活性剂后均使它的水解速率常数Kobsd降低。The hydrolysis of p-nitrophenyl picolinate (PNPP) catalyzed by La3+-H2O2 compound was studied at pH :7.00,T=30±0.1℃ in this paper.The effect of CTAB and polyoxy ethlene 23 lauryl ether (Brij35)surfactant on the hydrolysis of p-nitrophenyl picolinate (PNPP) catalyz-ed by La3+-H2O2 compound was also studied in this paper. The results indicated that the activated h-ydroxyl group of the compound effective nucleophilic species in the hydrolytic reactions.When the hydrolysis reaction was :=1:3, the result kobsd is up to maximum.

关 键 词：La(Ⅲ)-H2O2模拟酶 催化水解 PNPP 

分 类 号：O643.12[理学—物理化学]