明胶酶B及其抑制物与大鼠肾脏衰老关系的研究  

THE SIGNIFICANCE OF IMBALANCE BETWEEN GELATINASE B AND TISSUE TPYE INHIBITOR OF METALLOPROTEINASE-1 DURING THE PROCESS OF AGING IN KIDNEY OF RATS

作　　者：魏丛军[1] 高波[1] 

机构地区：[1]江苏省苏北人民医院肾脏内科,江苏扬州225001

出　　处：《实用临床医药杂志》2004年第5期31-34,38,共5页Journal of Clinical Medicine in Practice

基　　金：国家"973"资助项目 (G2 0 0 0 0 5 70 0 3 ) ;国家自然科学基金"创新研究群体"资助项目 (3 0 12 10 0 5 )

摘　　要：目的　探讨明胶酶B(MMP 9)及其抑制物 (TIMP 1)在大鼠肾脏衰老过程中的作用。方法　选用 3月、12月、2 4月龄大鼠 ,采用免疫组化技术分别检测MMP 9、TIMP 1、Ⅲ型胶原 (ColⅢ )、纤连蛋白 (FN)、增殖细胞核抗原 (PCNA)及α 平滑肌肌动蛋白 (α SMA)在不同鼠龄大鼠肾组织中的表达。结果　TIMP 1主要表达在肾小球、小管间质及血管 ,并随增龄表达增强 (P <0 0 1) ;MMP 9、主要表达在肾小管上皮细胞 ,随增龄表达无变化 ;ColⅢ主要表达在肾小球及小管间质 ,随增龄表达增强 (P <0 0 1) ;FN主要表达在肾小球及小管间质 ,随增龄表达增强 (P <0 0 1) ;α SMA除正常在血管平滑肌表达以外 ,在 2 4月鼠肾间质纤维化部位、肾小球系膜细胞及增厚的包曼氏囊有阳性表达 ;PCNA在 2 4月鼠肾小球、肾小管的细胞核及肾间质纤维化部位的成纤维细胞核有表达。TIMP 1与肾小球硬化有相关性 (P <0 0 5 )。TIMP 1与小管间质纤维化面积有相关性 (P <0 0 5 )。结论　MMP 9/TIMP 1表达失衡在大鼠肾脏衰老过程中可能起重要作用。Objective To investigate the expression and role of Gelati na se B (MMP-9) and tissue type inhibitor of metalloproteinase-1 (TIMP-1) in the aging process of rats kidney.Methods Immunohistochemical ass ays were applied to detect the expression of TIMP-1, MMP-9, collagensⅢ(Col Ⅲ ), fibronectin(FN), proliferating cell nucleus antigen (PCNA), and (α-smooth m uscle actin (α-SMA) in renal tissue of rats. The rats were divided into 3 grou ps(n=8): three-month-old group(3 m), twelve-month-old group (12 m) and t wenty-four-month-old group (24 m).Results TIMP-1 was dete cted in tubulointerstitia, periglomerular and perivascular regions, and increase d signinificantly with aging (P<0.01). MMP-9 was detected in tubular epit helial cells. No change of the expression of MMP-9 was found among the 3 groups . The expression of CoⅢ was signinificantly increased in renal tissue of 24 m r ats (P<0.01). The expression of FN was signinificantly increased in renal tissue of 24 m rats (P<0.01). α-SMA was normally detected only in vascul ar walls, but also detected in mesangial cells, thickened Bowman, capsule and in terstitium of 24 m rats. PCNA positive cells were found in tubulointers titial cells and tubular epithelial cells of renal tissue of 24 m rats. TIMP-1 had positive correlations with glomerulos clerosis (P<0.05). TIMP-1 had positive correlations with interst itial fibrosis area(P<0.05).Conclusion The imbalance be tween metalloproteinases and tissue type inhi-bitor of metalloproteinases may p lay an important role during the process of aging in kidney of rats.

关 键 词：衰老 肾脏 明胶酶B 抑制物 肾小球硬化 肾小管 

分 类 号：R339.33[医药卫生—人体生理学]