中国红鲤四群体线粒体DNA遗传多样性、起源及分化  

Mitochondrial DNA genetic diversity, origin and evolution of four populations of red common carps in China

作　　者：王成辉[1] 李思发[1] 邹曙明[1] 

机构地区：[1]上海水产大学农业部水产种质资源与养殖生态重点开放实验室,上海200090

出　　处：《水产学报》2004年第6期640-644,共5页Journal of Fisheries of China

基　　金：上海水产大学浙江龙泉市合作项目"瓯江彩鲤种质特性与开发利用研究"

摘　　要：用13种限制性内切酶对兴国红鲤、玻璃红鲤、荷包红鲤及瓯江彩鲤4种红鲤的线粒体DNA(mtDNA)进行RFLP分析。共产生17种限制性态型,其中5种酶为限制性片段长度多态性(RFLPs),归结为5种单倍型;兴国红鲤、玻璃红鲤、荷包红鲤和瓯江彩鲤的核苷酸多样性(π)分别为0.0087、0.0059、0.0094、0.0286,瓯江彩鲤的遗传多样性最为丰富;瓯江彩鲤起源于单倍型II为主的母系祖先,推算分化时间分别约在11.5万年、9.5万年前;玻璃红鲤和荷包红鲤起源于单倍型I为主的母系祖先,推算分化时间分别约为5～5.6万年、2万年前。RFLP analysis of mtDNA from Xingguo red common carp(C. carpio var. singuonensis), glass red common carp(C.carpio var.wananensis), purse red common carp(C.carpio var.wayuanensis) and Oujiang color common carp(C. carpio var. color) was conducted by 13 restriction endonucleases. 17 restriction morphs in all red carps were detected and in which 5 morphs were polymorphic and were defined as 5 kinds of haplotypes. The average nucleotide diversity (π) in Xingguo red common carp, glass red common carp,purse red common carp and Oujiang color common carp was 0.0087, 0.0059, 0.0094 and 0.0286 respectively, which indicated there was the greatest genetic diversity in Oujiang color carp. Oujiang color carp would have originated from a maternal ancestor mainly composed of haplotype II, 115 thousand and 95 thousand years ago respectively. Glass red carp and purse red carp would have originated from a maternal ancestors mainly composed of haplotype I, 50-65 thousand and 20 thousand years ago respectively.

关 键 词：兴国红鲤 玻璃红鲤 荷包红鲤 瓯江彩鲤 线粒体DNA 遗传多样性 起源 分化 

分 类 号：Q343.35[生物学—遗传学] S917[农业科学—水产科学]