Livin mRNA的反义核酸诱导MCF-7乳癌细胞凋亡作用(英文)  

Induction effects of antisense phosphorothioate oligodeoxynucleotides of livin mRNA on apoptosis in MCF-7 cells

作　　者：张华东[1] 袁守军[1] 陈惠鹏[1] 田增月[1] 韩昌明[1] 

机构地区：[1]军事医学科学院放射医学研究所药理室,北京100850

出　　处：《中国临床药理学与治疗学》2004年第12期1353-1356,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的 :以livinmRNA为靶点设计反义硫代脱氧寡核苷酸 (PS ODNs) ,探讨其体外抗肿瘤效应及其机制。方法 :设计以livinmRNA为靶点的反义核酸并作用于MCF 7乳癌细胞 ,用MTT、RT PCR和流式细胞仪检测等方法对其进行评价研究。结果 :在设计的一些反义核酸中 ,YMZ0 5能够有效地抑制livin基因的表达 ,增加caspase 3的活性 ,诱导MCF 7细胞凋亡 ,明显抑制其生长 ,结论 :通过抑制livin基因的表达能够抑制MCF 7乳癌细胞生长、诱导其凋亡 ,livin可能会成为抗肿瘤的新靶点。AIM: To design antisense phosphorothioate oligodeoxy nu cleotides (PS-ODNs) which targeted to livin mRNA and investigat e the anti-canc er effects and the mechanism in vitro. METHODS: Several antisense PS-ODNs were designed and exposed to MCF-7 breast cancer cel ls. MTT, RT-PCR, flow cytometry and caspase-3 assay were tested in the experim ent. RESULTS: The level of livin mRNA was decrea sed and the acti vity of caspase3 was increased by YMZ05 in several PS-ODNs designed. The growth of MCF-7 was inhibited and the cell apoptosis was induced by PS-ODN. CONCLUSION: The inhibition of livin can inhibit the grow th of MCF-7 bre ast cancer cells and induce the apoptosis of it. The livin may b ecome a new potential target of anticancer therapy.

关 键 词：反义 磷酰硫代脱氧寡核苷酸 LIVIN MRNA MCF-7细胞 细胞凋亡 

分 类 号：R966[医药卫生—药理学]