重组人白细胞介素10对角朊细胞增殖及产生细胞因子的影响  

Effects of recombinant human interleukin-10 (rhIL-10) on proliferation and cyt okines production of normal human keratinocytes

作　　者：陈飞虎[1] 陈敏珠[2] 李俊[1] 汪光宝[1] 智强[3] 

机构地区：[1]安徽医科大学药学院 [2]安徽医科大学药学院临床药理研究所 [3]安徽桑尼生物技术研究所

出　　处：《中国临床药理学与治疗学》2004年第12期1377-1380,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的 :研究重组人白细胞介素 10 (rhIL 10 )对无血清培养的角朊细胞增殖和产生细胞因子的影响 ,探讨其治疗银屑病的作用机制。方法 :用四甲基偶氮唑蓝比色法 (MTT)测定其对细胞增殖的影响 ;小鼠胸腺细胞增殖法检测白细胞介素 1(IL 1) ;ELISA法检测白细胞介素 6 (IL 6 )、白细胞介素 8(IL 8)。结果 :重组人白细胞介素 10抑制角朊细胞增殖与IL 1、IL 6及IL 8的分泌 ,并呈剂量依赖关系。结论 :重组人白细胞介素 10抑制角朊细胞与细胞因子分泌 ,可能是治疗银屑病的作用机理之一。AIM: To investigate the effects of recombinant human in terleukin-10 (rhIL-10) on proliferation and cytokines production of serum-fre e cultured normal human keratinocytes stimulated by PMA and lipopolysaccharides( LPS). METHODS: The influences of rhIL-10 on the proliferation o f serum-free cultured normal human keratinocytes were assayed by MTT. Interleuk in-1, interleukin-6, and interleukin-8 was detected by Enzyme-linked immunos orbent assay (ELISA). RESULTS: The rhIL-10 significantly inhibi ted the proliferation and the production of IL-1, IL-6 and IL-8 by KC which w as activation by PMA/LPS with dose-dependent manner. CONCLUSION: The rhIL-10 inhibits the proliferation and cytokines production of s erum-free cultured normal human keratinocytes. The anti-psoriatic effects of r hIL-10 may be related to inhibiting hyperplasia of epidermal cells, promoting t he formation of granular cells and possessing combined anti-inflammatory and im munomodulatory activity.

关 键 词：重组人白细胞介素10 角朊细胞 细胞因子 银屑病 

分 类 号：R966[医药卫生—药理学]