天青Ⅰ共振光散射法测定DNA  

Determination of deoxyribo nucleic acid with azure I by reconance light scattering method

作　　者：曾金祥[1] 陈小明[1] 罗和安[1] 蔡昌群[1] 张丽[1] 

机构地区：[1]湘潭大学化学学院,湘潭411105

出　　处：《分析试验室》2004年第12期35-38,共4页Chinese Journal of Analysis Laboratory

基　　金：湖南省自科基金资助项目(00JJY2084);湖南省教育厅资助项目(03C611)

摘　　要：基于脱氧核糖核酸(DNA)对混合有机染料天青Ⅰ的共振光散射增强效应,拟定了一种测定DNA的共振光散射法。在pH9.5～10.5的范围内,天青Ⅰ在299、355、400、570、630nm附近均有较弱的共振光散射信号,随着DNA的加入,共振光散射信号大大增强。在355nm处,其散射光增强强度与DNA质量浓度呈线性关系。其线性回归方程为ΔI=-96.62+606.6ρ,线性范围为0 20～0.60μg mL,相关系数r=0.9998,检出限为11.2μg L。该方法可应用于合成样品中DNA的测定。The resonance light scattering(RLS) spectra of azure Ⅰ with deoxyribonucleic acid were studied. The RLS intensities were of azure Ⅰ was greatly enhanced by deoxyribonucleic acid in pH range of 9.5～10.5. There were resonance light scattering peaks at 299, 355, 400, 570 and 630 nm respectively.The enhanced intensity of RLS at 355 nm was proportional to the concentration of deoxyribonucleic acid. So a method of RLS for the determination of deoxyribonucleic acid was established. The linear equation for yDNA was ΔI=-96.62+606.6ρ(μg/mL). The linear range of the calibration curve was 0.20～0.60 μg/mL. The detection limit was 11.2 μg/L. This method was simple, rapid and was applied to the determination of DNA in synthetic samples with satisfactory results.

关 键 词：脱氧核糖核酸 天青Ⅰ 共振光散射法 

分 类 号：O657.32[理学—分析化学]