原子吸收法间接测定银杏黄酮  

Indirect determination of flavonoids of ginkgo biloba by atomic absorption spectroscopy

作　　者：上官小东[1] 郎惠云[1] 马亚军[1] 

机构地区：[1]西北大学化学系

出　　处：《分析试验室》2004年第12期48-50,共3页Chinese Journal of Analysis Laboratory

摘　　要：提出了间接测定银杏叶精提物中银杏黄酮的原子吸收光谱法。银杏黄酮与乙酸铅发生配合反应,生成难溶的棕黄色沉淀,经离心分离后,用原子吸收法测定上清液中过量的铅离子,可间接测定银杏黄酮。方法线性范围为5.0～30.0μg mL,RSD为1.6%～1.8%,回收率为100.3%～106.0%。An atomic absorption spectroscopic method was used for indirect determination of flavonoids of Ginkgo biloba via Pb(Ⅱ) precipitation. Using method, the flavonoids of Ginkgo biloba reacted with Pb^(2+) and led to brown-yellow precipitate. The precipitate was centrifuged and Pb^(2+) in supernatant was determined by AAS. The calibration curve was linear for flavonoids of Ginkgo biloba in the range from 5.0 to 30.0 μg/mL (r=0.9995), RSD=1.6%～1.8% and the recovery was 100.3%～106% (n=10). The method was applied to the determination of flavonoids of Ginkgo biloba with good results.

关 键 词：银杏黄酮 原子吸收光谱法 Pb(Ⅱ) 

分 类 号：O657.32[理学—分析化学]