A型全血加入O型全血后的血液流变学变化  

Experimental Hemorheology Study on Group A Whole Blood Added With Group O Whole Blood

作　　者：肖瑞卿[1] 赵树铭[1] 林武存[1] 曾杰[1] 陈英[1] 许汝福[2] 郭辉[3] 刘景汉[4] 

机构地区：[1]第三军医大学附属西南医院输血科 [2]第三军医大学军事预防医学院,重庆400038 [3]第三军医大学附属西南医院检验科,重庆400038 [4]中国人民解放军总医院输血科,北京100039

出　　处：《中国实验血液学杂志》2004年第6期833-836,共4页Journal of Experimental Hematology

基　　金：军队"十五"指令性课题;编号 0 1L0 45 -0 1

摘　　要：为了研究异型输血时A型受者接受大剂量O型全血的血液流变学 (红细胞计数、红细胞聚集指数、红细胞变形指数、红细胞刚性指数、全血还原黏度 )的变化 ,取 4℃保存 2 4小时的全血 ,于A型全血 60ml中分别加入 9、12、15、18mlO型全血 ,其量相当于 5 0公斤个体输入 60 0、80 0、10 0 0和 12 0 0ml血液 ,然后置 3 7℃孵箱 ,用摇摆器以 80次 /分钟摇动混匀。分别在 3 0分钟、2、4、8、12和 2 4小时留取标本 ,使用FASCO—系列 3 0 2 0B型全自动血流仪进行血液流变学检查。结果表明 :不同比例混合后不同比例间的红细胞计数、聚集指数、变形指数、刚性指数、全血还原黏度无显著性差异 ,混合血液不同时间红细胞聚集指数无差异 ,其它 4项指标有显著性差异。结论 :A型全血与O型全血最大混合达 60∶18时 ,对混合后血液流变学指标无影响 ,推测 5 0公斤体重伤病员 ,快速输入O型全血 12 0 0ml短期内对红细胞功能无明显的损害。The study was to investigate the hemorheologyic changes of group A blood recipient tranfused with large amounts of group O whole blood, by erythrocyte count, sympexis index, erythrocyte deformation index, erythrocyte rigid index and whole blood reduced viscosity, 60 ml of group A whole blood were added with 9, 12, 15 and 18 ml of group O whole blood (which corresponds to the the 4 000 ml whole blood added with 600, 800, 1 000 and 1 200 ml whole blood). The mixed blood was incubated at 37℃ with mixing at 80 times per minute. Samples were taken from the mixed blood at 30 minutes, 2, 4, 8, 12 and 24 hours after culture, and the hemorheology of the mixed whole blood was determined by FASCO series type 3020B automatic rheograph apparatus. The results showed that there were no differences of erythrocyte count, sympexis index, erythrocyte deformation index, erythrocyte rigid index and whole blood reduced viscosity among all different kinds of mixed whole blood, and there was no difference of sympexis index at different times, but there were obvious differences of erythrocyte count, erythrocyte deformation index, erythrocyte rigid index and whole blood reduced viscosity. It is concluded that blood transfusion of 1 200 ml group O whole blood to a recipient with 50 kg of body weight but with different blood type in emergent situation may exert no harm to the erythrocytes of recipient in a short term.

关 键 词：异型输血 A型全血 O型全血 血液流变学 

分 类 号：R457.1[医药卫生—治疗学] R457.11[医药卫生—临床医学]