腾冲热海眼镜泉粉红色菌藻席的细菌组成分析  

Analysis of Bacterial Composition of The Pink Mat from Spectacles Hot Spring in Tengchong by Culture-independent Approach

作　　者：张东华[1] 李沁元[1] 刘杨[1] 彭谦[1] 

机构地区：[1]云南大学云南省微生物研究所教育部微生物资源开放研究重点实验室,昆明650091

出　　处：《微生物学报》2004年第6期820-823,共4页Acta Microbiologica Sinica

基　　金：国家自然科学基金资助项目 ( 3 9960 0 0 3 ;3 0 3 60 0 0 4)~~

摘　　要：应用免培养法系统研究眼镜泉粉红色菌藻席的细菌组成。经过克隆筛选 ,测定了 2 3个克隆的 16SrDNA插入片段的近全序列。与GenBank的序列进行比对和相似性分析 ,结果表明 ,组成该菌藻席的细菌分属于Proteobacte ria、Firmicutes、Bacteroidetes、Actinobacter、Deinococcus_thermus、Aquificals 6个类群 (phylum) ,表现出了高度的细菌多样性。结合分析温度相近的 5个热泉 :Octopusspring、HaegindiandFluidirspring、Olkelduhals、Grendalurspring中的菌藻席细菌组成 ,表明生态位相近的不同环境中 ,其物种组成相近。The bacterial composition of the pink mat was studied by culture-independent approach. 23 complete 16S rDNA sequences were obtained. According to the sequences alignment and analysis of comparability, the bacteria of the pink mat was consisted of Proteobacteria,Firmicutes,Bacteroidetes,Actinobacter,Deinococcus-thermus,Aquificals. And compared with bacterial composition of the mats from Octopus spring in Yellowstone Park and Haegindi and Fluidir spring,Olkelduhals,Grensdalur spring in Iceland, the pink mat in spectacles spring had highest bacterial diversity among them because it perhaps included lots of bacteria at lower temperature. And the result indicated the same community lived in the same niche and Aquficales was dominant group among bacterial composition of the mat in higher temperature and near-neutral hot spring.

关 键 词：热泉 菌藻席 细菌多样性 生态位 群落结构 

分 类 号：Q938[生物学—微生物学]