溶液中细胞色素b6f的原子力显微镜和扫描隧道显微镜观测  

Observation of Cytochrome b_6f in near-physiological conditions using AFM and STM

作　　者：李辉[1] 苏贵金[2] 李宾兴[3] 李晓东[1] 林毅[2] 万立骏[2] 李良璧[3] 韩宝善[1] 

机构地区：[1]中国科学院物理研究所磁学国家重点实验室,北京100080 [2]中国科学院化学研究所,北京100080 [3]中国科学院植物研究所光合作用与环境分子生理学重点实验室,北京100093

出　　处：《电子显微学报》2004年第6期658-662,共5页Journal of Chinese Electron Microscopy Society

基　　金：国家重点基础研究发展规划资助项目(批准号:G199801 0102).

摘　　要：本文用原子力显微镜(atomicforcemicroscope,AFM)和扫描隧道显微镜(scanningtunnelingmicroscope,STM)在Tricine NaOH OG缓冲溶液中研究了细胞色素b6f(Cytochromeb6f,Cytb6f)在固体表面上的吸附形态。首先用AFM观察了吸附在云母表面上的Cytb6f复合体,观察到了分散较好的蛋白质颗粒。进一步用高分辨率的电化学STM(EC STM)观察了吸附在Au(111)表面上的Cytb6f分子,在浓度较低时发现了其二聚体的吸附形态,而在浓度较高时Cytb6f分子在Au(111)表面聚集成薄膜。The absorption forms of Cytochrome b_6f (Cyt b_6f) on solid surface were investigated by atomic force microscopy (AFM) and scanning tunneling microscopy (STM) in Tricine-NaOH-OG solution. Well-dispersed granules of Cyt b_6f on mica surface were observed by AFM. High-resolution STM images of Cyt b_6f on Au (111) surface indicate the existence of Cyt b_6f dimers in near-physiological conditions,and with the rise of concentration the protein forms membrane on the Au (111) surface.

关 键 词：细胞色素 b6f 二聚体 原子力显微镜 扫描隧道显微镜 

分 类 号：Q-3[生物学]