大苞栝楼发根在光辐射发根反应器中的培养  

Cultivation of the Hairy Roots of richosanthes Bracteata Voigt in the Pho tobioreactor

作　　者：徐明芳[1] 黄伟建[2] 葛湘峰[2] 陈心满[2] 

机构地区：[1]暨南大学生物工程系,广东广州510632 [2]暨南大学水生生物研究所,广东广州510632

出　　处：《华南理工大学学报（自然科学版）》2004年第12期16-19,24,共5页Journal of South China University of Technology(Natural Science Edition)

基　　金：广东省科技攻关项目 (2KM0 2 5 0 1G) ;广东省科学基金资助项目 (980 0 2 2 )

摘　　要：利用农杆菌 (Agrobacteriumrhizogenes)R16 0 1诱导药用植物大苞栝楼 (Trichosan thesbracteataVoigt)外植体无菌苗发根 ,建立毛状根生物反应器培养系统 .分析了光照强度、溶氧饱和强度及碳源等外界影响因子对反应器中发根生长的作用 .结果表明 :在光照强度为 2 5W /m2 、、溶氧饱和强度为 80 %时 ,发根的比生长速率与生物量维持在较高的水平 ;蔗糖对发根生长的促进作用明显优于葡萄糖 :在 30～ 4 0g/L的糖含量范围内 ,于MS培养基中采用两级添加方式添加蔗糖 ,发根生物量 (9.5g/L)比采用单级添加方式的 (7.2g/L)高出 2 0 %以上 .Agrobacterium rhizogenes R1601 was used to induce the hairy roots from cotyl edons and stems of Trichosanthes bracteata Voigt, The cultivation system for the growth of hairy roots was established in a photobioreactor. The effects of light intensity, dissolved-oxygen saturation and carbon source on the growth of hairy roots were then investigated. The results show that the specific growth r ate of hairy roots maintains higher level when the dissolved-oxygen saturation and light intensity are respectively controlled at 80% and 25 W/m2. It is als o shown that sucrose sources affect remarkably growth of hairy roots than glucos e. When the sucrose concentration is controlled at 30～40 g/L, the biomass in t h e two grades culture system(9.5 g/L) is 20% more than biomass of hairy roots in the single culture system(7.2 g/L).

关 键 词：大苞栝楼 发根 农杆菌 光生物反应器 生长 培养 

分 类 号：Q944.6[生物学—植物学]