5种光合细菌种间原生质体融合及优良农用融合子的筛选鉴定  

Interspecif Protoplast Fusion of Photosynthetic Bacteria and Identification of the Fusants

作　　者：刘勇 张德咏 谭新球 罗源华 何明远 戴建平 张战泓[2] 

机构地区：[1]湖南省植物保护研究所,中国湖南长沙410125 [2]湖南省蔬菜研究所,中国湖南长沙410125

出　　处：《生命科学研究》2004年第4期344-350,共7页Life Science Research

基　　金：国家"十五"重大科技攻关项目(2002BA516A08);国家农业科技成果转化基金(2002430050603);湖南省杰出中青年基金专项(01JZY2099);湖南省自然科学基金(00JJY2017)

摘　　要：处于对数生长期的光合细菌球形红假单胞菌(Rhodopseudomonassphaeroides)、沼泽红假单胞菌(Rhodopseudomonaspalustris)、嗜酸红假单胞菌(Rhodopseudomdnasacidophila)、深红红螺菌(Rhodospirarubrum)、万尼氏红微菌(Rhodomocrobiumvannielii),经溶菌酶(3mg/L)处理50min后,获得了它们的菌体形成的原生质体,其再生率分别为80%、71%、82%、61%、74%.取等量的亲本菌株在35%的PEG(MW6000)诱导下两两融合5min,共10种组合.其融合率为球×沼2.5×10-4、球×嗜2.1×10-4、球×深2.0×10-4、球×万2.1×10-4、沼×嗜2.8×10-4、沼×深2.4×10-4、沼×万2.6×10-4、嗜×深2.0×10-4、嗜×万2.3×10-4、深×万2.4×10-4.经影印法鉴定:形成的融合子可以分别生长于以相应的有机物为唯一碳源的培养基上,所有融合子体积均相当于两亲本株体积之和,融合子菌落形态特征介于两亲本株之间.从中随机挑选100个融合子,以辣椒苗作为靶标植物,从上述融合子中筛选到了1株具有显著促进作物生长、提高抗病性的融合子.The regeneration rates of protoplasts for Rhodopseudomonas sphaeroides,Rhodopseudomonas palustris,Rhodopseudomdnas acidophila,Rhodospira rubrum,Rhodomocrobium vannielii were obtained,which were 78%,85%,73%,64%,73%,64%respectively,when the concentration of lysozyme was 3 mg/L in EDTA solution and two parent strains were at logarithmic growth stage. One fusant PSB F36 was collected which can regulate the growth of chilli rapidly. The frequency of the fusion is 2.5×10 4 as the 35%PEG(MW 6 000) induced the fusion reaction for 5 minutes.The fusants can grow on the mediums containing both propionate and Mannitol. The volumes of the fusant is 3.81μm3 which is approximately equal to the total volumes of 2 parent cells. The colony of fusant shared the characteristics of PSB 3 and PSB 6. The fusant can be applied as a candidate for producing micro biofertilizers.

关 键 词：球形红假单胞菌(球) 沼泽红假单胞菌(沼) 嗜酸红假单胞菌(嗜) 深红红螺菌(深) 万尼氏红微菌(万) 原生质体融合 融合子的鉴定 

分 类 号：Q939.9[生物学—微生物学]