酵母双杂交筛选与RSA14-44相互作用的蛋白  

Screening of the protein interacting with RSA-14-44 using yeast two hybrid method

作　　者：袁莉刚[1] 田永强[2] 刘英[1] 缪时英[2] 王琳芳[2] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]中国协和医科大学分子生物学国家重点实验室,北京100005

出　　处：《甘肃农业大学学报》2004年第6期679-682,共4页Journal of Gansu Agricultural University

基　　金：国家重点基础研究发展规划项目(G1999055901)

摘　　要：构建了包含RSA14-44cDNA的pAS2-1-14诱饵质粒,并进行了人睾丸cDNA文库的酵母双杂交筛选,获得了一条编码人20S蛋白酶体蛋白PSMB5的cDNA片段;用所获得的序列编码该蛋白3’端编码框内全部氨基酸(191个氨基酸);首次证明RSA14-44与PSMB5在酵母细胞中存在相互作用。RSA14-44 is a new gene located on the fourth chromosome of rat and obtained from the rat testis tissue. To obtain the protein interacting with RSA14-44, the recombinant bait plasmid pAS2-1-14 was constructed and transformed into yeast strain Y190 to test pAS2-1-14 for no autonomous activation. One true positive, which obtained by screening the Human testis cDNA libraries, was confirmed to be PSMB5 by sequencing and protein BLAST. It concluded that RSA14-44 could interact with PSMB5.

关 键 词：RSA-14-44 酵母双杂交 PSMB5 

分 类 号：Q786[生物学—分子生物学]