花生遗传转化影响因素研究  

Research of Influence Factors on Peanut Transformation Mediated by A. tumefaciens

作　　者：李春娟[1] 万书波[1] 许婷婷[1] 庄伟建[2] 单世华[1] 

机构地区：[1]山东省花生研究所,山东青岛266100 [2]福建农林大学作物科学学院,福建福州350002

出　　处：《花生学报》2004年第4期20-25,共6页Journal of Peanut Science

基　　金：农业部"948"(2003 T12)项目;山东省农科院博士后基金项目

摘　　要：研究表明,农杆菌介导花生外源基因遗传转化过程中影响转化效率的因素较多。本文在实现花生外源基因遗传转化的基础上,针对所利用的丛生芽、胚轴和子叶外植体等受体材料,就遗传转化中涉及的预培养时间、侵染时间、共培养时间等处理因素对转化效率的影响进行深入探讨,以探索提高花生遗传转化效率的方法和途径,为该领域研究提供理论依据和技术参考。The study has previously transformed the binary antifungle genes, Chitinase and β-1,3-Glucnase gene, mediated with A. tumefaciens into peanut cultivars, Quanhua 10 and Jinhua 1012( Arachis hypogaea L.). The results showed that the regeneration system of three kinds of peanut explants were partially modified and complemented in this research, including advantitious shoots( As), somatic embryogenesis( Se), embryo leaflet( El). Transformation efficiency could be affected by the pre-cultivation time. Transformants have not yet been got through Se and El transformation ways but several scores of Km-resistance buds have been obtained and needed following induction and assaying, and the better transformation efficiency was seen with 10～20 min invasion in A. tumefaciens liquid and 72hr co-cultivation after it. Meanwhile, A. tumefaciens pollution could not be controlled easily when invasion and co-culture time was prolonged. So, how to get higher transformation efficiency but lower pollution ratio should be discussed in the future. In addition, there were some differences between the two used peanut cultivars during transformation.

关 键 词：花生 遗传转化效率 受体材料 预培养 子叶外植体 外源基因 农杆菌介导 影响因素 因素 共培养 

分 类 号：S565.2[农业科学—作物学] S513