不同方法建立动脉粥样硬化大鼠模型的比较  

Comparison of various atherosclerotic rat models

作　　者：沈丽[1] 卢维晟[1] 姚俊宇[2] 方根强[1] 王一尘[1] 

机构地区：[1]上海第二医科大学新华医院老年科,上海200092 [2]上海第二医科大学新华医院病理科,上海200092

出　　处：《心脏杂志》2005年第1期18-20,共3页Chinese Heart Journal

摘　　要：目的:比较3种不同方法建立的动物模型,探索建立造型简便且重复性好的大鼠动脉粥样硬化模型。方法健康雄性SD大鼠(体质量150～170g)24只,按3种方法建立模型:①高脂造模组,高脂饲料9周;②维生素D3造模组,维生素D340万单位灌胃3d,标准饲料9周;③高脂维生素D3造模组,维生素D320万单位灌胃3d,高脂饲料9周。采用HE染色鉴定各组的动脉粥样硬化病变形成情况。结果:光镜下高脂组血管各层结构基本正常;维生素D3造模组出现典型动脉粥样病变的斑块结构;高脂维生素D3造模组血管壁钙化和平滑肌细胞增生,程度较维生素D3组减轻,未出现典型斑块结构。结论:40万单位维生素D3的造模方法能在较短时间内建立典型成熟的动脉粥样硬化斑块模型。AIM: To investigate the simple and successful method for establishing atherosclerotic rat model . METHODS: Three groups of rats were obtained: high-fat fed (6 SD rats with high-fat diet for 9 weeks) ;vitamin D-treated (9 SD rats treated by 400,000 unit vitamin D for three days, common diet for 9 weeks );vitamin D-treated and high-fat fed (9 SD rats fed by 200,000 unit vitamin D for 3 days and high-fat diet for 9 weeks)。HE staining was used to verify the atherosclerotic lesion of three models. RESULTS: high-fat fed rats almost had no morphologic change in light microscope, while vitamin D-treated rats existd typical atherosclerosis. Vitamin D-treated and high-fat fed rats also showed semblable but much milder pathologic changes in light microscope . CONCLUSION:Method of 400,000 unit vitamin D for three days, common diet for 9 weeks is convenient and stable to establish typical atherosclerotic rat model .

关 键 词：模型 动脉粥样硬化 大鼠 病理学 

分 类 号：R541.4[医药卫生—心血管疾病]