聚乙烯缩丁醛膜固定聚亚甲基蓝和纳米银修饰的葡萄糖生物传感器  

Glucose biosensor based on poly(methylene blue) and Ag colloid modified glassy carbon electrode

作　　者：刘颜[1] 柴雅琴[1] 钟霞[1] 孙爱丽[1,2] 唐点平[1] 戴建远[1] 袁若[1] 

机构地区：[1]西南师范大学化学化工学院分析科学研究所,重庆400715 [2]安阳师范学院化学系,河南安阳455002

出　　处：《化学传感器》2004年第4期25-29,共5页Chemical Sensors

基　　金：国家自然科学基金(29705001);教育部优秀青年教师资助计划(教人司2002-40);重庆市自然科学基金(20027477)资助

摘　　要：用循环伏安法在玻碳电极上电聚合一层稳定的亚甲蓝聚合物膜,研究了这层膜在0.1 mol/L磷酸缓冲溶液(pH 7.0)中的电化学性质。用纳米银颗粒吸附葡萄糖氧化酶(GOD),采用乙烯醇缩丁醛(PVB)为辅助固酶基质将其固定于亚甲蓝修饰的玻碳电极表面,制成了新型葡萄糖生物传感器。实验发现,加入纳米银后提高了酶电极对葡萄糖的电流响应,所制备的传感器具有响应快、灵敏度高、稳定性好,对葡萄糖的线性响应范围为2.5×10^(-6)～2×10^(-3)mol/L,检测下限为1×10^(-6)mol/L,并具有抗坏血酸、抗尿酸干扰的特点。A new approach to construct an amperometric biosensor was described. A stable electropolymerized methylene blue films are electropolymerized on the glassy carbon by the method of cyclic voltammetry, their electrochemical quality is studied extensively in 0.1mol/L phosphate buffer (pH 7.0) . Glucose oxidase (GOD) had been adsorbed to colloidal silver sols combined with polyvinyl butyral (PVB), which were immobilized onto the surface of poly(methylene blue) modified glassy carbon electrode to prepare glucose biosensor. The results obtained showed that the Ag colloid could increase the current response to glucose and the biosensor exhibited fast response, high sensitivity, fine stability and linear dependence on the analyte concentration in the range of 2.5×10^(-6)～2×10^(-3) mol/L, with calculated limit of 1×10^(-5) mol/L. In the meantime the low operating potential of the biosensor can effectively eliminate interference from common interferents, such as ascorbic acid and uric acid.

关 键 词：丁醛 纳米银 聚乙烯 磷酸 新型 聚合物膜 PVB 固定 亚甲蓝 葡萄糖生物传感器 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]