大鼠孤束核在五羟色胺介导的胰腺外分泌中的作用  

Role of nucleus tractus solitarii in pancreatic secretion induced by 5-hydroxytryptamine in rats

作　　者：夏青[1] 李兆申[1] 屠振兴[1] 龚燕芳[1] 满晓华[1] 

机构地区：[1]中国人民解放军第二军医大学长海医院消化内科,上海市200433

出　　处：《世界华人消化杂志》2004年第12期2805-2808,共4页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30270504~~

摘　　要：目的:探讨在五羟色胺(5-HT)介导的胰腺外分泌中,大鼠孤束核所起的作用,并进一步阐明与这些作用相关的神经物质. 方法:在十二指肠内恒流灌注0.86 mol/L NaCl、10-4mol/L 5-HT,然后对各处理组中不同的孤束核切面(孤束核嘴侧平面、中间平面、尾侧平面)进行c-Fos免疫组化、c-Fos- NK1-R双重免疫组化染色方法(免疫荧光-免疫酶学),同时计数其中c-Fos阳性细胞数,并行定量分析;另外每15min 收集胆胰混合液一次,测定胰液蛋白含量. 结果:在孤束核各平面,5-HT组、0.86 mol/L NaCl组内c-Fos阳性神经元数量均高于假手术组(P<0.01),且在孤束核尾侧平面5-HT组(22.00±1.80)明显高于0.86 mol/L NaCl组(18.5±1.7)(P<0.01),另外两组在孤束核内的c- Fos阳性表达密集区域内均有NK1-R表达,而假手术组则未见c-Fos与NK1-R很好的重叠.胰蛋白测定方面:与假手术组胰液蛋白相比,5-HT组以及0.86 mol/L NaCl组在实验进行60 min(灌注后15 min)至135 min均有明显升高, 有统计学意义(P<0.01);且5-HT组(29.6±1.4 mg/15 min) 与0.86 mol/L NaCl组(18.1±2.4 mg/15 min)相比较,胰蛋白含量增加更为明显(P<0.01). 结论:在5-HT介导的胰液蛋白分泌中,大鼠孤束核起着感知及整理信息的作用,且这种作用的发挥与P物质受体有关.AIM: To investigate the role of nucleus tractus solitarii (NTS) in pancreatic secretion induced by 5-hydrox-ytryptamine (5-HT) in rats and to further elucidate the related neural substance. METHODS: Twenty-four rats were randomly divided into NaCl group, 5-HT group and sham operation group. Rats in NaCl and 5-HT groups were given NaCl (0.86 mol/L) and 5-HT (10-4 mol/L) via duodenum respectively and the mixture of bile and pancreatic juice was collected every 15 min. Protein content of the mixture was measured with BCA protein assay. EnVision Immunohistochemical method and double-label immunohistochemistry (immunoenzyme-fluorenscence) were used to detect the quantity of c-Fos and NK1-R positive cells in intermediate-medial NTS (mNTS), rostral (rNTS) and caudal regions (cNTS). RESULTS: The number of c-Fos positive cells was significantly increased in NaCl and 5-HT group as compared to that in sham operation group in rNTS, mNTS and cNTS (P<0.01), and in cNTS, the number was significantly larger in 5-HT group than that in NaCl group 22.00±1.80 vs 18.50±1.71, P<0.01). Moreover, NK1-R was expressed in the regions where c-Fos expression was enhanced by duodenal stimulation of NaCl and 5-HT, but this did not happen in sham operation group. After infusion of NaCl and 5-HT, pancreatic protein secretion was increased (P<0.01). And the content of pancreatic protein was significantly higher than that in 5-HT and NaCl groups (29.6± 1.4 mg/15 min vs 18.1±2.4 mg/15 min,P<0.01). CONCLUSION: NTS plays a role of sensing and processing information on pancreatic protein secretion induced by 5-hydroxytryptamine, in which the receptor of P substance may take a part.

关 键 词：大鼠 孤束核 五羟色胺 胰腺外分泌 免疫组化染色 

分 类 号：Q487[生物学—生理学]