烧伤后增生性瘢痕组织中基质金属蛋白酶2mRNA表达的临床研究  

Clinical investigation in the expression of matrix metalloproteinase 2 mRNA in the hyperplastic scar in burn patients

作　　者：杨勇[1] 韦多[1] 夏照帆[1] 

机构地区：[1]第二军医大学长海医院烧伤科,上海200433

出　　处：《中华烧伤杂志》2005年第1期43-45,共3页Chinese Journal of Burns

基　　金：国家自然科学基金资助项目(39570721)

摘　　要：目的观察烧伤后增生性瘢痕组织中基质金属蛋白酶2(MMP2)的动态表达情况。方法术中切取23例深Ⅱ度烧伤患者创面自然愈合后形成的早、中、晚期增生性瘢痕组织23块。采用原位杂交法检测MMP2mRNA在各期瘢痕组织中的表达和分布,并计算MMP2的阳性细胞百分比。结果MMP2主要由瘢痕组织中的成纤维细胞表达。早期瘢痕组织中MMP2mRNA阳性表达少,其阳性细胞百分比为(13.9±3.6)%;中期瘢痕组织中MMP2mRNA阳性表达增多,其阳性细胞占(68.7±5.2)%;晚期瘢痕组织中MMP2mRNA表达极低,其阳性细胞仅占(2.0±0.9)%。结论MMP2与瘢痕组织重塑过程密切相关。Objective To investigate the dynamic expression of matrix metalloproteinase 2 in the hyperplastic scar in burn patients. Methods Twenty-three pieces of hyperplastic scar tissue samples were harvested from the patients with deep partial thickness burn wound during the early, middle and late periods after natural wound healing. In situ hybridization technique and positive cell percentage counting were employed to determine the expression and the distribution of MMP-2 mRNA in the scar tissue from different periods with that of the middle period as the control. Results MMP-2 mRNA was mainly expressed in the fibroblasts in scar tissue. The positive percentage of MMP-2 in early period was 13.9±3.6 %, and it increased thereafter, till it reached 68.7±5.2%in the middle period. However, it declined to 2.0±0.9% in the late period. Conclusion MMP-2 might be closely related to the process of scar tissue remodeling.

关 键 词：MMP-2 瘢痕组织 增生性瘢痕 阳性细胞 基质金属蛋白酶2 MRNA表达 烧伤 成纤维细胞 

分 类 号：R644[医药卫生—外科学]