高氧液对大鼠烫伤后肠黏膜下调蛋白质的影响  

Influence of hyperoxic fluid on the down-regulated proteins of intestinal mucosa in scalded rats

作　　者：王晓军[1] 孙永华[2] 王建宁[1] 丁勤学[3] 张莉萍[4] 宋国成[1] 肖宏[1] 

机构地区：[1]北京市第六医院综合外科,100007 [2]积水潭医院烧伤科 [3]北京师范大学生命科学学院细胞生物学研究所 [4]北京军区总医院科研科

出　　处：《中华烧伤杂志》2005年第1期60-62,共3页Chinese Journal of Burns

基　　金：国家重点基础研究发展规划资助项目(G199905420)

摘　　要：目的观察高氧液对烫伤大鼠肠黏膜下调蛋白质的影响,为伤后早期应用高氧液治疗提供理论依据。方法制作大鼠35%TBSAⅢ度烫伤模型,设烫伤后普通补液组(烫伤组)、伤后输入高氧液为干扰因素组(高氧液组)和正常对照组,每组6只。利用高分辨双向电泳(2DE)对肠黏膜组织进行蛋白质分离,采用Imagemaster2DElite图像分析软件进行分析,应用生物质谱、蛋白质库及文献分析等方法研究高氧液对大鼠烫伤后肠黏膜下调蛋白质的影响。结果烫伤组大鼠明确下调的34个蛋白质点中,高氧液组出现明确上调的蛋白质点数为9。它们是线粒体乌头酸酶、丙酰辅酶A羧化酶、短链羟酰基辅酶A脱氢酶、雌二醇安息香酸盐转录因子、磷酸丙糖异构酶1、T淋巴细胞受体Vδ6和类动力蛋白5。结论高氧液可以上调大鼠严重烧伤后早期肠黏膜中的下调蛋白质,进而部分恢复肠黏膜的屏障功能。Objective To investigate the influence of hyperoxic fluid on the down-regulated proteins of intestinal mucosa in scalded rats, so as to provide a theoretical basis for the clinical use of hyperoxic fluid. Methods Spregue-Dawley (SD) rats with 35% TBSA full-thickness scald were randomly divided into scald control (S, n=6,with intraperitoneal fluid infusion after scalding), hyperoxic (H, n=6, with hyperoxic fluid infusion after scalding)groups. Six rats without scald injury served as normal group. The proteins in the intestinal mucosa were separated with the two-dimensional electrophoresis (2-DE), and were analyzed with ImageMaster 2D Elite. The influence of hyperoxic fluid on the down-regulated proteins of intestinal mucosa in scalded rats was studied with bio-spectrum, protein bank and document analysis. Results (1) Among the 34 down-regulated protein spots in S group, 9 of them definitely exhibited up-regulation compared with those in H group. (2)The expression of mitochondrial aconitase, alpha-propionyl-CoA carboxylase, short chain of hydroxylacyl-Coenzyme A dehydrogenase, transcription factor EB(estradiol benzoate), triosephosphate isomerase 1, T cell receptor V delta 6, and dynein-like protein-5 in H group were significantly up-regulated. Conclusion The hyperoxic fluid could up-regulate the down-regulated proteins in rat intestinal mucosa at early postburn stage, so that the barrier function of intestinal mucosa of rats with severe burns could be partially recovered.

关 键 词：高氧液 烫伤 肠黏膜 大鼠 蛋白质 辅酶A 治疗 生物质谱 动力蛋白 T淋巴细胞受体 

分 类 号：R644[医药卫生—外科学] R574[医药卫生—临床医学]