生物脱硫菌根癌土壤杆菌UP-3的固定化研究  

Study on Immobilization of the Desulfurizing Bacteria Agrobacterium tumefaciens UP-3

作　　者：张建辉[1] 侯影飞[1] 俞继仙[1] 孔瑛[1] 

机构地区：[1]石油大学华东化学化工学院,山东东营257061

出　　处：《生物技术》2005年第1期65-68,共4页Biotechnology

基　　金：中国石油天然气集团公司青年创新基金资助项目(W0 0 0 4 2 4 );石油大学 (华东 )博士创新基金资助项目 (No .B2 0 0 4 - 0 6)

摘　　要：生物脱硫催化剂固定化研究对生物脱硫技术的推广应用具有重要的意义。该文以筛选出的具有脱硫能力的根癌土壤杆菌UP - 3为固定化研究对象 ,二苯并噻吩 (DBT)为生物催化脱硫的模型化合物 ,主要考察了菌株UP - 3的培养条件、固定化方法和载体、固定化操作条件和固定化细胞的使用条件。结果表明 :以桑特斯培养基在 30℃下培养 2 8h的根癌土壤杆菌UP - 3具有最佳活性。采用 3wt%海藻酸钠水溶液为包埋载体 ,液菌比为 2 0∶1,在 4℃下 1wt%CaCl2 水溶液中固定化 2 4h,得到的固定化细胞脱硫性能最好。在 30℃下 ,反应 6d可将浓度为 6 2 5mg L的DBT降解 6 0Study on immobilization of the desulfurizing bacteria is very important for the application of biodesulfurization technology.In this paper,Agrobacterium tumefaciens UP-3 which had desulfurizing capabilities was studied using dibenzothiophene as a model compound.The culturation conditions,immobilization method and carrier,immobilization conditions and the conditions for application of the immobilized cell were mainly studied.The results indicated the cell growing at 30℃ by 28 hours had the highest activity,the immobilized cell had the optimum desulfurization effect under the conditions of 3% SA as carrier,1% CaCl_2 as precipitator,4℃ for immobilization temperature and 24 hours for immobilization time.At 30℃,more than 60%DBT at the concentration of 625mg/L was degraded after 6 days.

关 键 词：生物脱硫 固定化 海藻酸钠 二苯并噻吩 

分 类 号：Q939[生物学—微生物学]