丽格海棠的组织培养  

Tissue Culture in Rieger Begonia

作　　者：李海燕[1] 徐永清[1] 樊锐锋[1] 胡宝忠[1] 

机构地区：[1]东北农业大学生命科学学院植物教研室,黑龙江哈尔滨150030

出　　处：《生物技术》2005年第1期79-81,共3页Biotechnology

摘　　要：由于丽格海棠通过扦插获得的量有限 ,进口种子的价格又很昂贵 ,所以以它的叶、茎、花茎、叶柄等营养器官作为外植体 ,进行组织培养来快速获得丽格海棠。通过诱导芽分化和诱导愈伤两种途径 ,研究不同植物生长调节剂及浓度 ,对外植体萌发和生根的影响。研究表明基本培养基为MS ,最佳分化培养基为 6 -BA 1.0 0mg L +NAA 0 .10mg L ;最佳生根培养基为 1 2MS +NAA 0 .10mg L ;花土∶沙子 =1∶1,是丽格海棠最佳的驯化移栽基质 ,在该基质中生根苗移栽成活率达 90 %Because quantity of Begonia Rieger is limited and price of import seed is expensive,leaves,bud,flower petiol and petiole of vegetative organ of Begonia Rieger,who were used as tissue culture explants,form plant of Begonia Rieger.By the two pathway of inducing bud differentiation and wound,study on different plant growth regulator and concentration affect germination of explant and root.The result shows that the base culture medium is MS.The appropriate culture medium were (1) MS+6-BA1.00 mg/L+NAA0.10 mg/L for germination;(2) MS+ 6-BA0.50 mg/L+ NAA0.10 mg/L for rapid propagation;( 3) 1/2MS+NAA0.50 mg/L and 1/2MS+ IBA0.10 mg/L for rooting.The appropriate transplantationstroma is earth offlower and sand.The survival rate of transplanted was to 90%.

关 键 词：丽格海棠 组织培养 快速繁殖 生长调节剂 

分 类 号：Q813.1[生物学—生物工程]