板蓝根提取纯化及浓缩干燥工艺的研究  

Studies on Extraction and Purifying Process for Radix Isatidis

作　　者：徐德然[1] 刘学华 黄罗生[1] 

机构地区：[1]中国药科大学中药制剂研究室,南京210038 [2]丽珠利民制药厂医药研究所,韶关512028

出　　处：《海峡药学》2004年第6期67-70,共4页Strait Pharmaceutical Journal

摘　　要：目的　优化板蓝根提取纯化及浓缩干燥的工艺条件。方法 　以靛玉红及总氮含量为指标 ,采用正交试验及比较试验 ,对回流、超滤、减压浓缩和干燥等工艺条件进行比较研究。 结果 　最佳提取条件为 :用 9倍量 60 %的乙醇回流提取两次 ,第一次提取 2 h,第二次 1.5 h;用分子截留值为 3万的超滤膜可把靛玉红保留在滤渣中 ;用 4倍和 2倍乙醇提取两次 ,每次 1h,可把保留在沉淀和滤渣中的靛玉红提取完全 ;浓缩温度对靛玉红含量没有显著影响 ,但浓缩体积和干燥温度对靛玉红含量有显著影响。 结论 　本工艺靛玉红提取转移率为 3 2 .86% ,是药典工艺的113 9.2 2 % ,总氮提取转移率为 46.5 2 % ,是药典工艺的 10 5 .0 0 % 。OBJECTIVE The purpose is to optimize the extraction purifying condensing and drying process for Radix Isatidis.METHODS The process was studied by ultrafiltration decompressing condensing and drying methods and orthogonal design with the content of indirubin and nitrogen as the detective markers.RESULTS The optimum extraction condition:adding 9 times amount of ethanol and then cooking for 2 hours respectively.The optimum ultrafiltration condition:ultrafiltration membranes with MWCO above 3000,cooking the deposition 2 twice with 1 h respectively,condemning in 60℃,drying in 40℃,the volume of condemning was as little as possible.The condensing temperature attached no remarkable influence on the content of indirubin but condensing volume and drying temperature did.CONCLUSION The extraction rate of indirubin is 32.86% in the process above which is ten times as much as the process in Pharmacopoeia while the nitrogen content is almost as sameas the process in Pharmacopoeia.This extraction process has good stability.

关 键 词：板蓝根 靛玉红 总氮量 提取工艺 超滤工艺 正交试验 

分 类 号：R284.2[医药卫生—中药学] TQ460.4[医药卫生—中医学]