抗角蛋白单克隆抗体用于羊水栓塞病例病理诊断的研究  

STUDY OF PATHOLOGICAL DIAGNOSIS FOR THE AMNIOTIC FLUID EMBOLISM WITH THE RESIST KERATINS MONOCLONAL ANTIBODY

作　　者：于家华[1] 吴国民 周玲[3] 王成海[1] 

机构地区：[1]扬州大学医学院,江苏扬州225001 [2]江苏省靖江市公安局,江苏靖江214500 [3]江苏省苏北人民医院,江苏扬州225001

出　　处：《实用临床医药杂志》2004年第6期29-30,33,共3页Journal of Clinical Medicine in Practice

基　　金：江苏省级重点实验室开放课题资助项目 (项目编号 :KJS0 3 0 48)

摘　　要：目的　研究羊水栓塞病例肺动脉及肺泡壁毛细血管中角蛋白阳性物质的表达 ,探讨其病理诊断的客观性指标。方法　应用抗角蛋白 16单克隆抗体对 14例羊水栓塞病例的肺组织切片进行免疫组织化学LSAB法染色 ,并以 2例正常人体皮肤组织为阳性对照 ,显微镜下观察其角蛋白表达情况。结果　 14例羊水栓塞病例的肺组织和 2例正常人体皮肤组织的切片中角蛋白免疫组织化学染色均表达为阳性。结论　免疫组织化学LSAB法抗角蛋白染色是确诊羊水栓塞客观而灵敏的方法。Objective To explor the objective index of pathological di ag nosis for amniotic fluid embolism by studying the expression of the positive mat erial of keratin in the capillary vessel of alveolus wall and pulmonary artery. Methods The expression of the keratin in 14 cases of amniotic fluid embolism lung tissue was observed by immunohistochemical technique (LSAB method) with resist the keratin 16 monoclonal antibody, and positive expression control group with the skin tissue of 2 normal persons. Results All cases were positive expression by immunohistochemical technique that invo lved lung tissue of 14 cases of amniotic fluid embolism and skin tissue of 2 nor mal human. Conclusion It was an objective and sensitive metho d of pathological diagnosed of the amniotic fluid embolism by immunohistochemica l technique (LSAB method) with the resist keratin.

关 键 词：羊水栓塞 单克隆抗体 角蛋白 免疫组织化学 病理诊断 

分 类 号：R714.25[医药卫生—妇产科学]