长爪沙鼠生化基因位点检测方法的建立  

Establishment of Monitoring Methods of Biochemical Markers in Meriones Unguiculataus

作　　者：孙贺娟[1] 王冬平[1] 王钜[2] 李桂军[1] 陈振文[2] 

机构地区：[1]军事医学科学院实验动物中心,北京100071 [2]首都医科大学实验动物科学部,北京100054

出　　处：《上海实验动物科学》2004年第4期211-213,217,254,共5页Shanghai Laboratory Animal Science

基　　金：国家自然科技资源共享学者重大项目 2003DEA3N033)北京248工程科技重大项目(H020220050390)

摘　　要：为建立长爪沙鼠生化基因位点检测方法,应用蛋白质及同功酶醋酸纤膜电泳法,对长爪沙鼠 的生化基因位点进行了筛选,并摸索了长爪沙鼠同工酶和蛋白质的电泳条件。结果共筛选出适合长 爪沙鼠常规遗传检测的生化基因位点28个,确定了适当的电泳时间、电压和靶器官。该方法可为 长爪沙鼠的遗传质量分析提供有效手段。To establish monitoring methods of biochemical markers in Meriones Unguiculataus. the biochemical markers of Meriones Unguiculataus were screened , and its electrophoretic conditions of isoenzymes and proteins were probed according to cellulose acetate membrane electrophoresis of proteins and isoenzymes. A total of 28 suitable biochemical markers were screened out as regular genetic monitoring for Meriones Unguiculataus, and appropriate tissue, run time and voltage of electrophoresis were determined. The Monitoring methods of biochemical markers established may be useful for genetic quality analysis of Meriones Unguiculataus.

关 键 词：长爪沙鼠 生化 基因位点 靶器官 检测方法 筛选 蛋白质 遗传检测 同功酶 电泳条件 

分 类 号：Q953[生物学—动物学]