神经生长因子对Wistar大鼠皮瓣成活及其血流容积值的影响  

Effect of Nerve Growth Factor on Promoting the Survival Rate and Flow Volume Value of Random Flap in Wistar Rat

作　　者：赵建文[1] 刘芬兰[2] 银欢[1] 张甦[3] 

机构地区：[1]沈阳医学院实验动物中心,辽宁沈阳110034 [2]沈阳医学院医学基础部人体解剖学教研室,辽宁沈阳110034 [3]沈阳医学院教务处,辽宁沈阳110034

出　　处：《沈阳医学院学报》2004年第4期219-220,237,共3页Journal of Shenyang Medical College

摘　　要：目的 :研究神经生长因子 (nervegrowthfactor ,NGF)对Wistar大鼠皮瓣成活的影响。方法 :采用Wistar大鼠背部随意皮瓣动物模型 ,自身对照。手术后第 1d ,随机选择一侧皮瓣为实验组 ,皮下注射 0 5ml(6 6 7μg)NGF ;另一侧为对照组 ,皮下注射 0 5ml生理盐水。借助BPM2 镭射多普勒血液仪对随意皮瓣的血流容积值进行动态监测。结果 :实验组皮瓣成活率明显高于对照组 (P <0 0 5 )。实验组血流容积值 ,从术后第 2d起 ,在皮瓣蒂部 ,从术后第 5d起 ,在皮瓣中部、末端 ,显著高于对照组 (P <0 0 5 )。结论 :NGF能促进皮瓣血管的再通与增生 ,加速血液微循环的重建 ,提高随意皮瓣的成活长度 ,并可达到早期断蒂的目的。Objective:To study the function of nerve growth factor(NGF) on promoting the survival rate of the big-proportioned and super-lengthed random flap.Methods:The animal model of random flap was used in Wistar rat back with the comparison between the experimental group flap and the control group flap being in the same back.Subcutaneous injection with NGF 0.5 ml(66.7 μg) or normal saline 0.5 ml was applied locally in the experimental or control group flap on the first day after operation. The flow volume value of flap was monitored constantly with BMP2.Results:The survival rate of flap in the experimental group increased markedly(P<0 05) compared with that in the control group. From the 2nd day or the 5th day after operation,the flow volume values of various parts of flap in the experimental group were remarkably higher than those in the control group(P<0 05).Conclusion:NGF can promote the reconnection of the blood circulation of flap and increase the survival length of the random flap. Thus,the pedicel of flap can be cut off earlier.

关 键 词：皮瓣成活 血流 WISTAR大鼠 对照组 NGF 神经生长因子 皮下注射 容积 结论 实验 

分 类 号：R622.1[医药卫生—整形外科] R743[医药卫生—外科学]