兔缺血预适应心肌中环氧化酶-2的表达及阿司匹林的影响作用  

Expression of cyclooxygenase-2 in ischemic preconditioning myocardium and the effect of aspirin on prostaglandin metabolites in rabbit

作　　者：解玉泉[1] 陈大年[1] 王邦宁[1] 沈玉芳[1] 黄其植[1] 周青[2] 汪渊[2] 吴正升[3] 吴强[3] 

机构地区：[1]安徽医科大学第一附属医院心内科 [2]安徽医科大学分子生物学教研室,合肥230022 [3]安徽医科大学病理学教研室,合肥230022

出　　处：《安徽医科大学学报》2005年第1期46-49,共4页Acta Universitatis Medicinalis Anhui

摘　　要：　目的　观察兔晚期缺血预适应 (DPC)心肌中环氧化酶 2(COX 2)的表达量以及不同剂量阿司匹林对其影响。方法　新西兰兔 32只,随机分为 4组,DPC组(A组,n=8);正常对照组 (B组,n=8 );小剂量阿司匹林 ( 10mg/kg) +DPC组(C组,n=8);大剂量阿司匹林 (20mg/kg) +DPC组(D组,n=8)。结扎兔冠状动脉左前降支建立缺血预适应模型, 24h后取心肌组织,使用Western blotting及免疫组化方法分别测定兔DPC心肌中COX 2的表达。结果　兔DPC心肌COX 2表达量比对照组明显上升,差异有显著性 (P<0 01);实验前分别给予小、大剂量阿司匹林对COX 2表达量影响较小,差异无显著性(P>0 05)。结论　兔DPC心肌中COX 2明显表达,缺血预适应分别前予小、大剂量阿司匹林对COX 2表达量影响较小。Objective To observe the expression of cyclooygenase 2(COX 2) in delay ischemic preconditioning myocardium and the effect of Aspirin on prostaglandin metabolites in rabbits. Methods New Zealand rabbits( n =32)were randomly divided into 4 groups:group A( n =8):delay ischemic preconditioning, group B( n =8):normal controls,group C( n =8):low dosage aspirin(10 mg/kg)and delay ischemic preconditioning,group D( n =8):high dosage(20 mg/kg)aspirin and delay ischemic preconditioning. A mode of ischemic preconditioning myocardium was reproduced by ligation left anterior descending branch. After 24 hours, the COX 2 of myocardial section were investigated by Western blotting and immunohistochemistry. Results The expression of COX 2 in group A was significantly higher than in group B ( P <0 01).The levers of COX 2 were not higher in group C than that in group D and did not differ significantly from group B( P >0 05). Conclusion The COX 2 protein is expressed in delay ischemic preconditioning myocardium in rabbits.The COX 2 protein is not inhibited by high dosage aspirin on prostaglandin metabolites in rabbits as low dosage aspirin as well.

关 键 词：缺血处理 氧化还原酶类 

分 类 号：R973[医药卫生—药品] R977.3[医药卫生—药学]