高效液相色谱法测定瑞巴匹特的含量  

Determination of rebamipide by HPLC

作　　者：刘新元[1] 

机构地区：[1]天津隆顺榕制药厂,天津300232

出　　处：《天津药学》2005年第1期15-16,共2页Tianjin Pharmacy

摘　　要：目的 :建立高效液相色谱法测定瑞巴匹特含量的方法。方法 :采用 C1 8柱 (2 5 0 mm× 4 .6 mm,5 μm) ,以甲醇 -醋酸 /醋酸钠缓冲溶液 (49∶ 5 1)为流动相 ,流速为 1.0 ml/min,检测波长为 32 7nm。结果 :瑞巴匹特浓度在 0 .1～ 0 .6mg/ml范围内 ,线性关系良好 ,r=0 .9999。回收率为 99.5 % ,RSD为 0 .8% (n=5 )。结论 :本方法简便、准确、专属 ,可用于本品的含量测定及质量控制。Object: To establish an HPLC method to determine the contents of rebamipide. Methods: A C_ 18 column (250 mm×4.6 mm,5 μm) was used. The mobile phase consisted of methanol-acetic acid/sodium acetate buffer solution (49∶51). The flow rate was 1.0 ml/min and the detection wavelength was 327 nm. The analysis was performed at room temperature. Results: The standard curve for rebamipide was liner in the range of 0.1 to 0.6 mg/ml (r=0.999 9). The average recovery was 99.5% and RSD was 0.8%(n=5). Conclusion: This method was convenient, sensitive and actuate, and suitable for the quality control.

关 键 词：高效液相色谱法 瑞巴匹特 含量测定 

分 类 号：R972.2[医药卫生—药品]