maspin基因启动区的初步鉴定  

Primary characterization of maspin promoter

作　　者：贺美兰[1] 姜安丽[1] 张鹏举[1] 陈蔚文[1] 刘志芳[1] 张建业[1] 

机构地区：[1]山东大学医学院生物化学教研室,山东济南250012

出　　处：《山东大学学报（医学版）》2005年第1期1-4,共4页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金资助项目(30171026)

摘　　要：目的:研究maspin基因表达的调控机制。方法:利用PCR技术扩增maspin基因5'上游启动区860bp(-765～+95bp)不同的缺失片段,并将它们分别与荧光素酶(LUC)报告基因相连,构建了6种maspin-LUC报告基因表达质粒,通过转染实验检测maspin启动区不同长度片段在雄激素依赖性前列腺癌细胞(LNCaP)和雄激素非依赖性前列腺癌细胞(PC-3M)中驱动荧光素酶表达的活性。并转染雄激素表达受体(AR)到PC-3M细胞,观察雄激素及其受体对不同片段表达活性的作用。结果:maspin基因启动区(-312～247bp)之间含有与雄激素受体相关的负性调控元件,在(+14～95bp)之间含有一个功能性的中文(Sp1)调控元件。结论:maspin基因的表达受雄激素受体及Sp1的调节。Objective: To study the mechanism of maspin gene transcriptional regulation. Methods: Different deleted fragments of maspin promoter were amplified by PCR and subcloned into the pGL3-basic vector respectively. By transfection experiment,the luciferase activities driven by 6 different fragments in androgen-dependent prostate cancer cells LNCaP and androgen-refractory prostate cancer cells PC-3M were detected. In addition,AR expression vector was transfected into PC-3M cells to investigate the effect of androgen and its receptor on the activity of maspin promoter. Results: There was a negative element related to androgen receptor in the distal region of (-312～247bp) in maspin promoter,and a functional Sp1 element in proximal fragment of +14～95bp. Conclusion: The expression of maspin gene is regulated by androgen receptor and Sp1.

关 键 词：基因 MASPIN 调控序列 核酸 前列腺肿瘤 

分 类 号：Q344.14[生物学—遗传学]