重组海葵肽类毒素hk2a对急性心功能不全犬左心功能的影响  

Effects of a novel recombinant polypeptide from the sea anemone Anthopleura sp. on left ventricular function in dogs with acute cardiac insufficiency

作　　者：欧阳平[1] 谢晋国[1] 刘俭[1] 肖文星[1] 刘彦波[1] 王磊[2] 梁东[2] 王永华[2] 许顶立[1] 刘伊丽[1] 徐安龙[2] 

机构地区：[1]南方医科大学南方医院心血管内科,广东广州510515 [2]中山大学生命科学学院国家高技术计划海洋生物功能基因组开放实验室,广东广州510275

出　　处：《第一军医大学学报》2005年第1期37-39,43,共4页Journal of First Military Medical University

基　　金：国家863计划项目(2001AA628090);广州市科委科技攻关引导项目(2002Z3-E4131;2002z3-C7111)~~

摘　　要：目的观察重组海葵肽类毒素(hk2a)对急性心功能不全犬左心功能的影响。方法采用右心室快速起搏法成功建立了犬的急性心功能不全动物模型,利用超声心动图评价静脉注射hk2a前后不同时间段急性心功能不全犬左心功能的变化情况,并设立对照组进行比较。结果hk2a能够在给药后即刻即显增加急性心功能不全犬左室射血分数(P<0.05),其作用时间持续30min以上(P<0.05)。与西地兰组相比具有起效快、作用强等特点。结论新型hk2a能够明显改善急性心功能不全犬的左心功能。Objective To observe the effects of recombinant hk2a, a novel neurotoxin from the sea anemone Anthopleura sp., on left ventricular function of dogs with acute cardiac insufficiency. Methods Canine models of acute cardiac insufficiency were established by rapid ventricular pacing, in which the left ventricular ejection fraction (LVEF) was measured by Acuson ultrasound systems (Sequoia 512) at 0, 5, 15, 30 and 60 min, respectively, after intravenous injection of 30 μg/kg recombinant hk2a. The response of the canine models to hk2a treatments was observed in comparison with that of the dogs treated with Cedilanid (as positive control) and saline (as negative control). Results Intravenous injection of recombinant hk2a caused an immediate and significant increase in LVEF in the canine models of acute cardiac insufficiency (P<0.05), and the effect maintained for more than 30 min without significant effect on heart rate. Recombinant hk2a possessed such merits as quicker onset and greater potency in comparison with Cedilanid. Conclusion Recombinant hk2a may significantly increase LVEF of the dogs with acute cardiac insufficiency.

关 键 词：心力衰竭/药物作用 海葵 正性肌力药物 

分 类 号：R541.6[医药卫生—心血管疾病]