木芙蓉叶有效组分对大鼠肾缺血再灌注损伤的保护作用(英文)  

Protection of the effective fraction of Hibiscus Mutabilis L.against injury of renal ischemic reperfusion in rats

作　　者：符诗聪[1] 罗仕华[1] 张凤华[2] 周玲珠[2] 

机构地区：[1]上海第二医科大学瑞金医院伤科,上海市伤骨科研究所,上海市200025 [2]上海第二医科大学瑞金医院生化室,上海市200025

出　　处：《中国临床康复》2005年第6期250-251,共2页Chinese Journal of Clinical Rehabilitation

基　　金：上海市教委资助重点课题(01B11)~~

摘　　要：背景:木芙蓉叶是我国著名伤科魏指薪教授的在临床应用于消非特异性炎症的非常有效的药物,已经有近60年历史,为了阐明该药的作用机制,以缺血再灌注损伤的模型进行研究,来说明其抗炎的作用机制。目的:观察木芙蓉叶有效组分(MFR)对大鼠肾缺血再灌注损伤中的保护作用,探讨MFR的抗炎作用机制。设计:以实验动物为研究对象的随机对照实验研究。单位:一所市级伤骨科研究所。材料:实验于2002-06/2003-06在国家中医药管理局重点实验室(上海市伤骨科研究所内实施完成),选取雄性Wistar大鼠55只。方法:采用大鼠肾缺血再灌注模型,木芙蓉叶有效组分灌胃,检测血清尿素氮、肌酐,并检测白细胞介素-1(IL-1)的含量。主要观察指标:①血尿素氮及肌酐的变化。②MFR对血清IL-1含量的影响。结果:经MFR治疗后,能明显改善大鼠缺血再灌注后肾功能的变化。假手术组大鼠血清尿素氮为(6.72±1.30)mmol/L,血清肌酐为(38.40±6.23)μmol/L。缺血1h并再灌注24h后,对照组大鼠尿素氮为(60.72±4.64)mmol/L,而MFR治疗组尿素氮为(47.34±8.32)mmol/L,两组之间的差异有显著性意义(t=2.562,P<0.05);治疗组和对照组的血清肌酐分别为(347.95±95)μmol/L和(518.20±41.15)μmol/L,两者之间差异具有显著性意义(t=3.69,P<0.01)。在IL-1的作用方面:肾?BACKGROUND:Hibiscus Mutabilis L.is a very effective Chinese traditional herb for non specific inflammation in the clinic adopted by Professor Wei Zhixin,the eminent expert in Traumatology Department in China, lasting for near 60 years.In order to explain its pharmaceutical mechanism, the research of Hibiscus Mutabilis L.was carried on in the model of ischemic reperfusion injury to explain its mechanism on anti inflammation. OBJECTIVE:To observe the protection of the effective fraction of Hibiscus Mutabilis L.(MFR) on renal ischemic reperfusion injury in rats to probe into the mechanism of MFR on anti inflammation. DESIGN:Randomized and controlled study on experimental animal. SETTING:A Municipal Institute of Traumatology and Orthopedic Department. MATERIALS:The experiment was performed in the Key Experiment Room(Institute of Traumatology and Orthopedic Department, Shanghai) of State Administration of Traditional Chinese Medicine, in which,55 male Wistar rats were employed. METHODS:The rat model of renal ischemic reperfusion was adopted and MFR was applied for gastric perfusion to determine serum blood urea nitrogen(BUN) and serum creatinine(Scr) and the level of interleukin 1(IL 1). MAIN OUTCOME MEASURES:① Changes in serum BUN and Scr;② Effect of MFR on level of serum IL 1. RESULTS:After MFR treatment,the renal function was improved remarkably after ischemic reperfusion in rats.In sham operation group, serum BUN was( 6.72± 1.30) mmol/L and serum Scr was ( 38.40± 6.23) μ mol/L.In 24 hours of reperfusion after one hour ischemia,BUN was( 60.72± 4.64) mmol/L in the control and( 47.34± 8.32) mmol/L in the MFR treatment group,and the significant difference presented between two groups(t =2.562,P< 0.05).Serum Scr was( 347.95± 95) μ mol/L and( 518.20± 41.15) μ mol/L in the treatment group and the control respectively, indicating significant difference(t=3.69,P< 0.01).Concerning to the effect on IL 1,in 1 hour of reperfusion after 1 hour renal ischemia,IL 1 was(122.79± 27.56)

关 键 词：木芙蓉叶 白细胞介素1 再灌注损伤 

分 类 号：R641[医药卫生—外科学]