糖尿病大鼠视网膜内转化生长因子-β_2及其受体的表达  

TGF-β_2 and its receptor expression in the retina of diabetic rats

作　　者：谢平[1] 刘庆淮[1] 戈应宾[2] 袁孝如[2] 

机构地区：[1]南京医科大学第一附属医院眼科,210029 [2]南京医科大学生理学系

出　　处：《江苏医药》2005年第2期110-112,共3页Jiangsu Medical Journal

基　　金：南京医科大学科技发展基金资助(NY02025)

摘　　要：目的　探讨糖尿病视网膜损伤和转化生长因子 β2 (TGF -β2 )产生的关系以及 TGF- β2的作用机制。方法　链脲佐菌素制备大鼠糖尿病模型,用 Western blot方法检测不同病程糖尿病动物模型(2 周及 20 周)视网膜中 TGF -β2 的表达,RT -PCR测定其受体 TGF- βRⅠ及 TGF βRⅡ的表达。结果　糖尿病大鼠视网膜内 TGF β2 随糖尿病进展表达增加,其受体 TGF βRⅠ的表达和对照组相比无显著差异,而TGF- βRⅡ的表达显著增加。结论　TGF- β2 在糖尿病视网膜病变(DR)形成过程中有重要作用,并有可能通过TGF- βRⅡ型受体起作用。Objective To investigate the relations between diabetic retinal damage and the generation of transforming growth factor-β 2(TGF-β 2) and the mechanism of the effect of TGF-β 2. Methods The diabetic rat model was established by injection of streptozotocin.The expression of TGF-β 2 in the retina of the diabetic rats at different stages(2w and 20w) was determined by Western blot method and its receptor TGF-βRⅠ and TGF-βRⅡ expression was measured by RT-PCR technique.Results The TGF-β 2 in the retina of diabetic rats increased with the progress of diabetes. Compared with the control, there was no obvious change in receptor TGF-βRⅠ expression, while the TGF-βRⅡ expression increased significantly.Conclusion TGF-β 2 plays a significant role in the development of diabetic retinopathy, and the effect might be taken through the receptor TGF-βRII.

关 键 词：表达 TGF-Β2 视网膜 Ⅱ型受体 转化生长因子-Β2 糖尿病大鼠 对照组 RT-PCR 果糖 链脲佐菌素 

分 类 号：R587[医药卫生—内分泌] R774[医药卫生—内科学]