β-磷酸三钙/聚L-乳酸与大鼠骨膜成骨细胞复合骨修复材料的研究  

Characteristics of β-Tricalcium Phosphate/Poly (L-lactic acid) Composite Combining with Rat Periosteum Derived Osteoblast

作　　者：周大利[1] 杨为中[1] 尹光福[1] 郑昌琼[1] 陈锐[2] 陈槐卿[2] 

机构地区：[1]四川大学材料科学与工程学院,成都610064 [2]四川大学生物医学工程研究室,成都610041

出　　处：《无机材料学报》2005年第1期105-111,共7页Journal of Inorganic Materials

基　　金：国家863高技术计划(2002AA326080)教育部优秀青年教师基金(2002123)四川省杰出青年基金(2001-2)

摘　　要：采用溶液浇铸-模压成型.沥滤方法制备了β-TCP／PLLA多孔支架材料,将支架材料与大鼠骨膜成骨细胞复合获得新型组织工程骨修复材料.通过抗压强度及压缩模量的表征研究了支架材料的力学性能;采用SEM观测、MTT法、碱性磷酸酶活性及骨钙素分泌量检测细胞复合材料的体外成骨特性;通过裸鼠肌袋种植,以组织学方法评价细胞复合材料的异位成骨能力.结果表明:β-TCP／PLLA多孔支架材料孔隙率可调,孔径为100-200μm,孔道相互贯通;材料抗压强度和压缩模量随孔隙率的增大而降低,β-TCP复合PLLA后材料的力学性能高于同孔隙率的纯PLLA多孔材料;复合支架材料适宜骨膜成骨细胞粘附和生长,无细胞毒性;骨膜成骨细胞复合β-TCP／PLLA支架材料的体外成骨特性良好,且具有体内异位成骨能力.β-TCP/PLLA porous carrier material was prepared by the improved process consisting of solvent casting, compression molding and leaching. A novel tissue engineering composite was developed by combining β-TCP/PLLA composite with rat periosteum derived osteoblasts. Mechanic properties of the composite carrier were determined with compressive strength and compressive modular measuring; Osteogenesis characteristics in vitro were evaluated by means of SEM examination, MTT assay, ALP activity and OCN content evaluation; Ectopic osteogenesis was investigated in muscle of nude mice by the histological method. Results show that β-TCP/PLLA porous structure has adjustable porosity and interconnected pores with 100-200 μm in size; Compressive strength and compressive modulus of the materials decrease sharply with porosity increasing. Mechanic properties of β-TCP/PLLA in low porosity are superior to those of PLLA alone with the same porosity; β-TCP/PLLA composite is fit for the adhesion and ingrowth of periosteum derived osteoblasts, and has no cytotoxicity; β-TCP/PLLA composite combined with periosteum derived osteoblasts possesses good osteogenesis potential in vitro and ectopic osteogenesis capacity in vivo.

关 键 词：Β-磷酸三钙 聚L-乳酸 骨膜成骨细胞 骨组织工程 

分 类 号：R318[医药卫生—生物医学工程] R329[医药卫生—基础医学]