动脉粥样硬化大鼠主动脉IL-1β、TNFα、IL-10及IL-10R的表达及银杏叶提取物的作用  

Expression of IL-1β,TNFα,IL-10 and IL-10R in aorta of atherosclerotic rats and effects of Ginkgo biloba extract on their expression

作　　者：焦亚斌[1] 芮耀诚[1] 杨鹏远[1] 李铁军[1] 邱彦[1] 

机构地区：[1]第二军医大学药学院药理学教研室,上海200433

出　　处：《第二军医大学学报》2005年第2期158-160,共3页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(30271509)

摘　　要：目的:研究动脉粥样硬化(AS)大鼠主动脉IL 1β、TNFα、IL 10及IL 10R的表达及观察银杏叶提取物(EGB)对它们的作用。方法:建立AS大鼠模型,动物分成3 组,即对照组、AS组和EGB组,每组6 只。EGB组每天灌胃给予EGB 100mg/kg,对照组、AS组每日给予同体积水。8周后,采用ELISA、RT PCR方法检测IL 1β、TNFα、IL 10 及IL 10R水平及mR NA表达。结果:AS大鼠主动脉IL 1β、TNFα、IL 10、IL 10R水平及mRNA表达均显著高于对照组(P<0.01),EGB组主动脉IL 1β、TNFα水平及mRNA表达均低于AS组,IL 10、IL 10R水平及mRNA表达均高于AS组(P<0.01)。结论:EGB对致炎细胞因子IL 1β、TNFα表达的显著抑制作用,对抗炎细胞因子IL 10及IL 10R表达的显著上调作用可能是其抗AS的机制之一。Objective:To study the expressions of IL-1β,TNFα,IL-10 and IL-10R in aorta of atherosclerotic(AS) rat and the effects of Ginkgo biloba extract (EGB). Methods:The experimental model of AS was established in rats. Rats were divided into 3 groups:control,AS and EGB. EGB (100 mg/kg) was administered through intra-gastric pathway in EGB group every day;the same volume of water was administered in control group and in AS group.After 8 weeks,the expressions of IL-1β,TNFα,IL-10 and IL-10R were detected by ELISA and RT-PCR methods. Results:The protein and mRNA expressions of IL-1β,TNFα, IL-10 and IL-10R in aorta were markedly higher in AS groups than that in control groups(P<0.01). The protein and mRNA expressions of IL-1β and TNFα in aorta were markedly lower in EGB groups than that in AS groups;the protein and mRNA expressions of IL-10 and IL-10R in aorta were markedly higher in EGB groups than that in AS groups(P<0.01). Conclusion:EGB has significant inhibitory effects on proinflammatory cytokine IL-1β and TNFα; upregulation of anti-inflammatory cytokine IL-10 and IL-10R by EGB may be partially responsible for its anti-AS effects.

关 键 词：动脉粥样硬化 细胞因子 白细胞介素10 银杏叶提取物 

分 类 号：R541.4[医药卫生—心血管疾病]