平阳霉素白蛋白微球诱导兔耳中央动脉闭锁的实验研究  

Effects of Pingyangmycin albumin microspheres on sclerostenosis of the rabbit central auricular arteries

作　　者：高庆红[1] 郑根建[2] 王晓毅[1] 周岚[2] 董佳增[1] 王昌美[1] 温玉明[1] 

机构地区：[1]四川大学华西口腔医学院颌面外科,四川成都610041 [2]浙江省台州市中心医院口腔科,浙江台州318000

出　　处：《上海口腔医学》2005年第1期42-47,共6页Shanghai Journal of Stomatology

基　　金：四川省科技攻关项目(03SG022-101);2002年浙江省台州市科委基金(022219)

摘　　要：目的:探讨平阳霉素白蛋白微球(PYM-AMS)诱导兔耳中央动脉闭锁的可能机制。方法:选择健康成年日本大耳白兔24只,采用区组随机法,在每只兔的双耳分别注射1号液(PYM水剂)、2号液(PYM油剂)、3号液(PYM-AMS油剂,为实验组)各0.26ml,PYM浓度为5mg/ml。每天观察注射药物后的动静脉变化,并于注射后2、7、14、21天处死6只动物,通过光镜和透射电镜观察兔耳中央动脉的组织学变化。结果:PYM水剂组血管壁和血管内皮细胞无明显改变;PYM油剂注入后约14天,血管充盈略下降,21天有血管内膜及血管内皮细胞增生;实验组,7～14天时,注药血管出现缩窄,但仍可见血流通过,远心端有小血栓。21天时,可见血管闭锁,小动脉有较小血栓,血流中断。光镜下,注射后2、7天,血管内皮细胞出现肿胀、脱落,小血管远心端可见微球栓塞。注射后14天,血管内皮细胞增生,血管壁增厚,管腔缩小;微球有吸收改变。注射后21天,动脉管壁增生明显,个别细胞核呈分裂像,血管腔闭锁;微球吸收明显,其远心端血管腔出现闭锁,小静脉内膜增生。经Masson和vanGieson染色证实,中央动脉增生血管壁为肌性细胞。透射电镜下,内弹性膜的管腔侧可见多层平滑肌细胞、成纤维细胞及肌纤维母细胞。结论:PYM-AMS通过栓塞和缓释PYM,可明显诱导兔耳中央动脉闭锁,PYM-AMS可用于动静脉畸形的治疗。PURPOSE: The aim of this study was to investigate the mechanism of Pingyangmycin-albumin microspheres(PYM-AMS) acted on the rabbit central auricular arteries. METHODS: In the study of randomized block design,24 Japanese white rabbits were divided into 4 groups,6 rabbits in each group. The animals were put on the operation table after being anaesthetized by intramuscular injection of Xumianxin(0.2ml/Kg) and the interior division was blocked to stop drug liquid get into the interior ear. After being sterilized with 75% alcohol,No.1(PYM hydrochloride injection + 0.9% NaCl),No.2(PYM + soybean oil),No.3(PYM-AMS + soybean oil) liquids which contained PYM 5mg/ml were injected into the central auricular arteries of the animals about 30 seconds(0.26ml/per ear),respectively. Then these vessels were examined histologically after 2,7,14,21 days respectively. RESULTS: After injection,in PYM+0.9% NS control group(No.1 liquid),the ears and vessels had no significant changes. In PYM+soybean oil control group (No.2 liquid),at the 2nd day,the endothelial cells were mild swollen. At the 7th day,some endothelial cells were dropped off. At the 14th day,the central auricular arteries had mild change,but the blood flow was not blocked. At the 21st day,the wall of the central auricular artery had more layers,especially on the intima,but the lumen was still obvious. A few of endothelial cells were proliferative. In PYM-AMS group(No.3 liquid),the blood was not resumed at once,and there was some oil in the vessels. At the 1st day,the injection site was mild swollen,and small thrombosis was observed in small vessels. At the 7th-14th day,the central auricular arteries were narrowed,but the blood could be observed. At the 21st day,the vessels had sclerostenosis,and the blood flow was blocked,but no scar and necrosis were observed. Under light microscopy,at the 2nd day,the endothelial cells were mild swollen and small vessels were embolized by PYM-AMS. At the 7th day,the endothelial cells were mild swollen. At the 14th day,the endothelial cell

关 键 词：血管畸形 平阳霉素 白蛋白微球 血管 栓塞 

分 类 号：R96[医药卫生—药理学]