11种天然药物对乳酸杆菌生长与产酸影响的体外研究  

In vitro study of the effect of 11 kinds of natural drugs on the growth and acid production of Lactobacillus

作　　者：黄正蔚[1] 周学东[2] 肖悦[2] 刘天佳[2] 李继遥[2] 

机构地区：[1]上海第二医科大学附属第九人民医院,口腔医学院口腔内科,上海200011 [2]四川大学华西口腔医学院口腔内科,四川成都610041

出　　处：《上海口腔医学》2005年第1期67-70,共4页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(30171175);'四川省教育厅国际合作项目基金(413020)

摘　　要：目的:研究11种天然药物对乳酸杆菌生长及产酸的影响。方法:以乳酸杆菌参考株AC413作为实验菌株,测定川芎、血藤、五倍子等11种天然药物的最小抑菌浓度(MIC)。再以低于MIC的4个浓度配制含药的TPY液体培养基,调定其初始pH为7.4,接种乳酸杆菌,厌氧培养后测定其终末pH。结果经单因素方差分析其统计学意义。结果:大黄、蜂房与儿茶在4.000mg/ml的浓度下即表现出明显的抑菌作用,而茶多酚及五倍子在8.000mg/ml时也表现出抑菌作用;但血藤、槟榔、川芎、黄芩、三七与白芷则对乳酸杆菌的生长无明显的抑制作用。茶多酚、蜂房、黄芩、五倍子和血藤对乳酸杆菌的产酸具有显著的抑制能力;川芎、大黄、槟榔和儿茶没有明显的影响;而三七和白芷对乳酸杆菌的产酸却有着一定的促进作用。结论:茶多酚、儿茶、五倍子、大黄和蜂房对乳酸杆菌的生长均有一定的抑制作用;茶多酚、大黄、蜂房、黄芩、五倍子和血藤还能抑制乳酸杆菌的产酸。PURPOSE: To study the effect of 11 different natural drugs on the growth and acid production of Lactobacillus,as a preparation for screening an effective agent to mediate the balance of oral microflora. METHODS: Lactobacillus AC413 was chosen as the experimental bacterium. Eleven kinds of drugs,such as Rhizoma Chuanxiong,Surgentodoxa cuneata and Galla Chinensis were extracted by means of maceration,percolation and reflux extraction. The values of MIC of various extracts were measured. Then,different experimental media containing various extracts were prepared. The concentration of the extracts was lower than the MIC of the drug and the initial pH of the medium was 7.4. Lactobacillus was cultured in the medium for 48 hours,and finally the rest pH was measured. One-way ANOVA was used for statistical analysis. RESULTS: When the concentration of the drugs was lower than 8.000mg/ml,Tea polyphenols,Catechu,Galla Chinensis,Radix et Rhizoma Rhei and Nidus Vespae can inhibit the growth of Lactobacillus effectively. Tea polyphenols,Nidus Vespae,Radix Scuteilariae,Galla Chinensis and Surgentodoxa cuneata can inhibit the acid production of Lactobacillus effectively,Rhizoma Chuanxiong,Radix et Rhizoma Rhei,Semen Arecae and Catechu have no preliminary effect on it,but Surgentodoxa Cuneata and Radix Angelicae Pubescentis can increase it. CONCLUSION: Tea polyphenols,Catechu,Galla Chinensis,Radix et Rhizoma Rhei and Nidus Vespae can inhibit the growth of Lactobacillus effectively,and Tea polyphenols,Radix et Rhizoma Rhei,Nidus Vespae,Radix Scuteilariae,Galla Chinensis and Surgentodoxa Cuneata can inhibit the acid production of Lactobacillus effectively.

关 键 词：天然药物 乳酸杆菌 生长 产酸 

分 类 号：R285[医药卫生—中药学]