棕色田鼠雄性幼体不同发育期犁鼻器和副嗅球的组织结构  

Histological Structure of the Vomeronasal Organs and Accessory Olfactory Bulbs of Male Mandarin Voles (Microtus mandarinus) at Different Postnatal Ages

作　　者：丁小丽[1] 邰发道[1] 

机构地区：[1]陕西师范大学生命科学学院,西安710062

出　　处：《兽类学报》2005年第1期57-62,共6页Acta Theriologica Sinica

基　　金：国家自然科学基金资助项目 ( 3 0 2 0 0 0 2 6) ;教育部科学技术重点项目

摘　　要：通过对出生后不同发育时期雄性棕色田鼠犁鼻器和副嗅球进行组织学观察 ,探讨棕色田鼠出生后犁鼻器和副嗅球的发育规律。实验以出生后当天 (0日龄 ) ,5日龄 ,15日龄 ,2 5日龄以及成年棕色田鼠为研究对象 ,副嗅球采用Pischinger氏染色法染色 ,犁鼻器用H .E .染色法染色后进行组织学观察。结果显示 ,棕色田鼠出生时 ,犁鼻器和副嗅球就已具有成体的基本结构 ,随着动物个体的发育 ,犁鼻上皮逐渐增厚 ,犁鼻管变长 ,犁鼻上皮中神经元密度增加 ;腺体逐渐增大 ,犁鼻管腔填充物增多 ,犁鼻管背外侧的静脉血管逐日增大 ,管腔周围出现越来越多的血管 ;副嗅球长宽都增加 ,僧帽细胞层和颗粒细胞层逐渐增长 ,各层细胞密度变化稍有不同 ;出生后 15日内 ,僧帽细胞层细胞密度增加 ,15日龄以后又开始降低 ,2 5日龄及成体的僧帽细胞层细胞密度与 5日龄的相似 ;颗粒细胞层细胞密度持续增高。实验结果提示 ,棕色田鼠 5日龄时 ,犁鼻器和副嗅球已具有了完整的结构 ,到 2We investigated the vomeronasal organ (VNO) and accessory olfactory bulbs (AOB) of male mandarin voles (Microtus mandarinus) at postnatal 0,5,15,25 days of ages using histological method. The VNO was stained using Pischinger method while AOB was stained using hematoxylin-eosin method. In the mandarin vole,which continues to grow during most of its adult life, VNO and AOB were well developed before birth. However, full development of VNO and AOB did not occur until postnatal 25 days. At birth, mandarin vole had the basic structure of VNO and AOB of the adults. From age 0 to age 25 days, the thickness of vomeronasal epithilium, the length of the vomeronasal duct, and the size of the gland increased with age. The dorsolateral vessel became larger and numbers of other vessels increased. The size of AOB, the length of the granule cell and the mitral cell zones, and the density of granule cells increased with age also, while the density of mitral cells increased from age 0 to age 15 days, then declined. Voles of age 25 days and the adults had the similar densities of mitral cells as those of age 5 days. These results suggest that possibly at postnatal age 5 days, the structure development of VNO and AOB was complete, and possibly at postnatal 25 days, VNO and AOB were fully functional.

关 键 词：副嗅球 棕色田鼠 雄性 细胞密度 成体 颗粒细胞 幼体 出生后 上皮 组织学观察 

分 类 号：Q959.837[生物学—动物学] Q426