几种生物学因子对薰衣草胚性愈伤组织悬浮培养生长的影响  

Effects of Several Biological Factors on the Growth of Embryogenic Callus Suspension Culture of Lavandula angustifolia, cv. Munstead

作　　者：许耀祖[1] 王晓军[1] 赵民安[1] 赵海清[1] 

机构地区：[1]中国科学院新疆理化技术研究所

出　　处：《生物技术通讯》2005年第1期34-36,共3页Letters in Biotechnology

基　　金：中国科学院西部行动高新技术资助项目(KGCX2-SW-506)

摘　　要：通过研究接种量、培养基、碳源、氮源及激素组合等生物学因子对薰衣草胚性愈伤组织悬浮培养生长的影响,建立了一种可快速生长的薰衣草胚性愈伤组织悬浮培养体系。结果表明,在添加0.05mg/L2,4-D、0.5mg/L6-BA、30g/L蔗糖及0.5g/L水解酪蛋白的MS培养液中,以50g/L的接种量进行薰衣草胚性愈伤组织的悬浮培养,其生长速率可达24.14g/(L·d)。Effects of several biological factors on the growth of embryogenic callus(EC) suspension culture of Lavandula angustifolia, cv. Munstead were studied, and a fast growing EC suspension culture system of Lavandula angustifolia was established. The results showed the EC growth rate could reach 24.14 g/(L·d) under the following biological conditions: the EC cultured on a Murashige and Skoog(MS) medium supplemented with 2,4-D 0.05 mg/L, 6-BA 0.5 mg/L, sucrose 30 g/L, and casein hydrolysate 0.5 g/L, with the amount of inoculation 50 g/L of fresh EC.

关 键 词：薰衣草 胚性愈伤组织 悬浮培养 接种量 培养基 碳源 氮源 激素 薰衣草油 

分 类 号：S573.9[农业科学—作物学]