沙眼衣原体D型MOMP基因的克隆和序列分析  

Cloning and identifying of complete major outer-membrane protein gene of chlamydia trachomatis serovar D

作　　者：杨章民[1] 韩俊宏[1] 郑瑾[1] 杨筱凤[1] 来宝长[1] 王一理[1] 司履生[1] 

机构地区：[1]西安交通大学生命科学与技术学院癌症研究所,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2005年第1期40-42,共3页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的　从D型沙眼衣原体培养物中克隆主要外膜蛋白(MOMP)基因。方法　利用细胞培养法扩增沙眼衣原体后,提取基因组为模板,经聚合酶链反应(PCR)扩增全长MOMP基因,并用酶切、PCR扩增及序列测定等方法对重组质粒进行鉴定。结果　成功扩增了MOMP基因,并插入克隆载体 pUCmT中。结论　MOMP基因的克隆为进一步开展沙眼衣原体的疫苗研究奠定了基础。Objective To clone target gene MOMP from cultured cells infected with chlamydia trachomatis serovar D. Methods The genome DNA of chlamydia trachomatis serovar D was extracted from cell culture and subjected to PCR as template. The MOMP gene was amplified and recombined with plasmid pUCmT. The recombinant plasmid pUCmT/MOMP was identified by restrictive enzyme digestion, PCR amplification and sequencing. Results The complete MOMP gene was cloned successfully and inserted into pUCmT plamid. Conclusion The target gene cloned from chlamydia trachomatis serovar D might provide a ready source for vaccine preparation.

关 键 词：沙眼衣原体 克隆 主要外膜蛋白 

分 类 号：R374[医药卫生—病原生物学]