HPLC-ELSD法测定乙肝宁颗粒中黄芪甲苷含量  

Determination of astragaloside in Yiganning Granules by HPLC-ELSD

作　　者：曹湘萍[1] 梁建宁[1] 何晓艳[1] 方磊[1] 

机构地区：[1]湖南省药品检验所,湖南长沙410001

出　　处：《中成药》2005年第2期165-167,共3页Chinese Traditional Patent Medicine

摘　　要：目的:建立高效液相色谱 蒸发光散射检测器法测定乙肝宁颗粒(黄芪、白花蛇舌草、党参等)中黄芪甲苷含量。方 法:采用AgilentC18(250mm×4.0mm,5μm)色谱柱;流动相:甲醇 水(75∶25);流速:1.0mL·min-1;检测器:SEDEX75。 结果:黄芪甲苷在0.5984～2.3936μg范围内具有良好的线性关系,回收率为95.41(RSD为1.1,n=5)。结论:本法 具有良好的精密度和重现性,结果准确可靠,是测定黄芪甲苷含量的较好方法。AIM: To establish a HPLC-ELSD method for determination of astragaloside in Yiganning Granules. METHODS: The HPLC-ELSD with the Agilent C 18 column (4.6 mm×250 mm, 5 μm) was used. The mobile phase was methanol-water (75∶25) at a flow rate of 1.0 mm·min -1 and the detector was SEDEX75. RESULTS: There was a good linear correlation in the range of 0.5984-2.3936μg. The recovery was 95.41% (RSD=1.1%, n=5). CONCLUSION: The method is proved to be precise、reproducibility and reliable. It is suitable for determination of astragaloside.

关 键 词：黄芪甲苷 乙肝 含量 HPLC-ELSD法 高效液相色谱-蒸发光散射检测器法 白花蛇舌草 RSD 颗粒 甲醇 重现性 

分 类 号：R284[医药卫生—中药学] R286.0[医药卫生—中医学]