9601颗粒对急性多发脑梗塞大鼠海马皮层MARCKS磷酸化调节作用的研究  

Study on regulating effects of 9601-Granules on phosphorization of MARCKS in hippocampal cortex in the rats with multiple acute cerebral infarct

作　　者：张允岭[1] 白文[2] 韩振蕴[1] 郑宏[1] 张綦慧[1] 石玉如[1] 金香兰[1] 张锦[1] 黄启福[3] 

机构地区：[1]北京中医药大学东方医院,北京100078 [2]北京大学人民医院,北京100044 [3]北京中医药大学基础医学院,北京100029

出　　处：《北京中医药大学学报》2005年第1期25-29,i001,共6页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.30171196);2003年国家人事部优秀留学回国人员重点项目基金;2004年教育部优秀青年教师基金资助

摘　　要：目的　研究急性多发脑梗塞大鼠海马皮层MARCKS及 p-MARCKS蛋白的表达,进一步观察9601颗粒对MARCKS信号转导通路的影响,从蛋白水平探讨中药对急性脑缺血保护作用机制。方法　采用改良Kaneko法建立急性多发脑梗塞动物模型,观察造模后各组大鼠的神经系统症状体征积分改变及海马皮层组织的病理变化及细胞超微结构的改变;应用免疫组织化学和固定化蛋白印迹法检测急性缺血后海马皮层MARCKS及 p -MARCKS蛋白表达的改变以及中药的调节作用。结果　MARCKS及p -MARCKS蛋白在急性缺血后海马皮层中表达比正常对照组明显升高(P<0 .05),应用9601和尼莫地平可以使海马皮层异常升高的MARCKS及 p -MARCKS表达下降,但中西药之间无显著差异(P>0. 05)。结论　在急性脑缺血状态下大鼠海马皮层MARCKS和 p- MARCKS蛋白表达异常升高,与缺血损害关系密切,9601颗粒对此具有明显的下调作用。Objective To study the expressions of MARCKS and p-MARCKS in the hippocampal cortex in the rats with multiple acute cerebral infarct, observe the effects of 9601-Granules on MARCKS signal transduction pathway, and investigate the protective mechanism of TCM drugs against acute cerebral ischemia on the basis of proteins. Methods The rat model of multiple acute cerebral infarct was established by using modified Kaneko method; changes in the scoring of symptoms and signs of nervous system, and changes in the pathology and cell ultrastructure of hippocampal cortex, were observed in each experimental group; changes in the expressions of MARCKS and p-MARCKS in hippocampal cortex experienced acute ischemia and the regulating effects of TCM drugs on their expressions were detected by using immunohistochemical method and Western blot method. Results The levels of the expressions of MARCKS and p-MARCKS in hippocampal cortex were higher in the group experienced acute ischemia than those in the normal control group (P<0.05). Although both 9601-Granules and Nimodipine could decrease the abnormal elevation in the expressions of MARCKS and p-MARCKS, there was no significant difference between the effects of the two drugs. Conclusion Under the condition of acute cerebral ischemia, the expressions of MARCKS and p-MARCKS are abnormally increased in hippocampal cortex in the rats, which is closely related with ischemic defect; and 9601-Granules possess marked downregulating effects on the abnormal increase.

关 键 词：急性多发脑梗塞大鼠模型 蛋白质 磷酸化 中药 9601颗粒 脑保护 

分 类 号：R285.5[医药卫生—中药学]