软骨细胞诱导骨髓基质细胞体内软骨形成  

In vivo chondrogenesis of BMSCs induced by co-transplantation of BMSCs and chondrocytes

作　　者：周广东[1] 王晓云[1] 刘天一[1] 苗春雷[1] 吕晓杰[1] 崔磊[1] 刘伟[1] 曹谊林[1] 

机构地区：[1]上海第二医科大学附属第九人民医院整形外科,上海第二医科大学组织工程中心,200011

出　　处：《中华实验外科杂志》2005年第3期272-274,i002,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目(30300353);国家"863"基金资助项目(2002AA205021)

摘　　要：目的探讨软骨细胞在体内非软骨形成部位促进骨髓基质细胞(BMSCs)向软骨分化并形成软骨的可行性。方法猪BMSCs与软骨细胞按一定比例(6∶4或7∶3)混匀,取2.5×107个混合细胞悬浮于0.5ml30%Pluronic溶液后注射到裸鼠皮下(n=6)。相同数量的单纯软骨细胞或BMSCs同样方法注射,分别作为阳性对照及阴性对照,0.75×107个软骨细胞同样注射作为低浓度软骨细胞对照。各组均8周后取材检测。结果混合细胞组及阳性对照组均形成了成熟的软骨。组织学可见成熟软骨陷窝、异染基质及Ⅱ型胶原表达。两组新生软骨糖胺多糖(GAG)含量差异无统计学意义(P>0.05),两混合组平均湿重分别为(320±48)mg和(294±37)mg,均达到阳性对照组70%以上。BMSCs组仅形成了纤维性组织,低浓度软骨细胞组在局部形成了少量软骨,但新生软骨平均湿重低于阳性对照的30%。结论上述结果提示软骨细胞能诱导BMSCs在体内非软骨形成部位向软骨分化并形成软骨组织。Objective To test the hypothesis that chondrocytes can promote in vivo chondrogenic differentiation and chondrogenesis of BMSCs at non-chondrogensis site.Methods Porcine BMSCs and auricular chondrocytes were mixed at different ratios (6∶4 or 7∶3) and 2.5×107 mixed cells were suspended in 0.5 ml 30% Pluronic F-127,and then the mixture was injected into nude mice subcutaneously as experimental groups.Chondrocytes or BMSCs at the same cell number were mixed with 0.5 ml Pluronic and injected respectively as controls.0.75×107 chondrocytes were mixed and injected as low concentration chondrocyte control.All samples were collected at 8th week post-injection.Results All specimens in experimental groups and chondrocyte group formed mature cartilage with collagen Ⅱ expression.Mature lacuna structures and metachromatic matrices were also observed in these specimens with the same level of GAG contents (about 10 mg/g).Average wet weight of specimens in experimental groups (320±48 mg and 294±37 mg respectively) was over 70% of that in chondrocyte group (418±33 mg).In contrast,specimens in BMSC group showed mainly fibrous tissue.Only a small amount of cartilage was formed in specimens of low concentration chondrocyte group and the average wet weight was below 30% of that in chondrocyte group.Conclusion Chondrocytes can promote in vivo chondrogenic differentiation and chondrogenesis of BMSCs at non-chondrogenesis sites.

关 键 词：软骨细胞 体内 软骨形成 骨髓基质细胞 BMSC 注射 混合细胞 阳性对照 软骨组织 表达 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]