钙池操纵的钙通道抑制剂对大鼠肝细胞钙池操纵的钙通道电流的影响  

The effects of 2-APB on store-operated Ca^(2+) channels currents in acutely isolated rat hepatocytes

作　　者：黄昌州[1] 张宗明 裘法祖[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院普外科,武汉430030 [2]清华大学第一附属医院普外科

出　　处：《中华实验外科杂志》2005年第3期311-312,共2页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金(30270532);教育部"跨世纪优秀人才培养计划"(200248);上海市教委"曙光计划"资助项目(02SG20)

摘　　要：目的探讨钙池操纵的钙通道(SOC)抑制剂(2APB)对大鼠肝细胞SOC电流(Isoc)的影响。方法急性分离大鼠肝细胞,应用全细胞膜片钳记录技术测定大鼠肝细胞Isoc,并观察2APB对该电流的影响。结果在急性分离的大鼠肝细胞记录到Isoc,从其电流电压(IV)曲线可以看出该Isoc的反转电位为-2.5mV左右。在钳制电位为-100mV时,该Isoc为(-664.52±140.44)pA(n=8);20、40、60、80、100μmol/L的2APB分别使Isoc由给药前的(-664.52±140.44)pA下降至给药后的(-564.80±111.01)、(-490.11±73.97)、(-362.01±55.91)、(-295.64±50.03)、(-232.12±46.47)pA,给药前后比较差异有统计学意义(P<0.05或P<0.01);2-APB的抑制作用呈浓度依赖性增强,其EC50为(64.63±10.56)μmol/L。结论2APB对急性分离的大鼠肝细胞Isoc具有显著的抑制作用,这对于进一步深入进行肝细胞钙超载的发生机制和防治具有重要意义。Objective To st,udy the effects of 2-APB on store operated calcium current (Isoc) in isolated rat hepatocytes.Methods (1) the isolation technique of rat hepatocellular,(2) whole cell patch clamp techniques were used to investigate the effects of 2-APB on store Operated calcium current (Isoc).Results (1)The peak amplitude of Isoc was ^-664.52± ~140.44 pA(n=8),its reveral potential was about ^-2.5 mV,(2)20,40,60,80,100 μmol/L 2-APB produced a concentration-dependent decrease of Isoc with IC_~50 value of 65.91±10.49 μmol/L (n=8);The peak value of Isoc in the I-V curve was decreased by 2-APB (n=8,~P< ~0.05 or ~P< ~0.01). Conclusion 2-APB had a protective effect on isolated rat hepatocytes,probably though inhibiting Isoc.

关 键 词：大鼠肝细胞 急性分离 钙通道电流 给药 抑制剂 影响 抑制作用 记录 依赖性 

分 类 号：R965[医药卫生—药理学]